43816

Sigma-Aldrich

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):
DTT, (±)-threo-1,4-Dimercapto-2,3-butanediol solution
Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein/REAXYS Number:
1719757
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

product line

BioUltra

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

3.0-5.0

density

1.04 g/mL at 20 °C

absorption

cut-off at 330 nm

suitability

in accordance for filter test

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Application

DL-dithiothreitol solution has been used:
  • in the treatment of intestinal explants
  • in homogenate preparation of gill cells for enzymatic assays
  • in western blotting
  • in transglutaminase activity

Biochem/physiol Actions

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Suppression of GABA B receptor function in vivo by disulfide reducing agent, dl-dithiothreitol (DTT)
Carai M A M, et al.
Psychopharmacology, 174(2), 283-290 (2004)
Delivery of human immunodeficiency virus vaccine vectors to the intestine induces enhanced mucosal cellular immunity
Wang L, et al.
Journal of Virology, 83(14), 7166-7175 (2009)
In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring
Dib A, et al.
Frontiers in physiology, 9, 350-350 (2018)
New Technologies, An Issue of Orthopedic Clinics (2018)
Expression and Localization of Cathepsins B, D, and G in Dupuytren?s Disease
Tan K, et al.
Plastic and reconstructive surgery. Global open, 6(2), e0133549-e0133549 (2018)
Articles
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