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443R-1

Sigma-Aldrich

Nanog (EP225) Rabbit Monoclonal Primary Antibody

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biological source

rabbit

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP225, monoclonal

description

For In Vitro Diagnostic Use in Select Regions

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (443R-14)
vial of 0.1 mL concentrate Research Use Only (443R-14-RUO)
vial of 0.5 mL concentrate (443R-15)
vial of 1.0 mL concentrate (443R-16)
vial of 1.0 mL concentrate Research Use Only (443R-16-RUO)
vial of 1.0 mL pre-dilute Research Use Only (443R-17-RUO)
vial of 1.0 mL pre-dilute ready-to-use (443R-17)
vial of 7.0 mL pre-dilute ready-to-use (443R-18)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (443R-18-RUO)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 (concentrated)

isotype

IgG

control

seminoma

shipped in

wet ice

storage temp.

2-8°C

visualization

nuclear

Gene Information

human ... NANOG(79923)

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conjugate

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antibody form

culture supernatant

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antibody form

affinity isolated antibody

antibody form

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clone

EP225, monoclonal

clone

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clone

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form

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species reactivity

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species reactivity

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species reactivity

human

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General description

Nanog is a transcription factor homeoprotein that is involved in maintaining embryonic stem cell pluripotency and self-renewal. Embryonic stem cells with a deleted Nanog gene lose pluripotency. Conversely, embryonic stem cells that are forced to differentiate will downregulate Nanog expression. Nanog expression has been shown to be absent in normal adult organ tissues, but positive expression in undifferentiated germ cell tumors such as seminoma, dysgerminoma and embryonal carcinoma has been noted. Therefore, Nanog may be used as an aid in the determination of undifferentiated tumors of germ cell origin from non-germ cell tumors.

Quality


IVD

IVD

IVD

RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ian Chambers et al.
Cell, 113(5), 643-655 (2003-06-06)
Embryonic stem (ES) cells undergo extended proliferation while remaining poised for multilineage differentiation. A unique network of transcription factors may characterize self-renewal and simultaneously suppress differentiation. We applied expression cloning in mouse ES cells to isolate a self-renewal determinant. Nanog
Martin C Chang et al.
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, 28(4), 347-355 (2009-06-02)
The embryonic stem cell transcription factors SOX2, NANOG, and OCT3/4 are involved in the regulation of germ cell tumor growth and differentiation. They, and D2-40 (podoplanin), an antigen expressed in seminomas, are emerging as useful diagnostic markers in testicular germ
Adam H Hart et al.
Cancer, 104(10), 2092-2098 (2005-10-06)
The NANOG gene, a member of the homeobox family of DNA binding transcription factors, was recently identified in a screen for pluripotency-promoting genes. NANOG overexpression in murine embryonic stem cells is sufficient to maintain self-renewal and to block differentiation. The
Guangjin Pan et al.
Cell research, 17(1), 42-49 (2007-01-11)
Several extrinsic signals such as LIF, BMP and Wnt can support the self-renewal and pluripotency of embryonic stem (ES) cells through regulating the "pluripotent genes." A unique homeobox transcription factor, Nanog, is one of the key downstream effectors of these
Kaoru Mitsui et al.
Cell, 113(5), 631-642 (2003-06-06)
Embryonic stem (ES) cells derived from the inner cell mass (ICM) of blastocysts grow infinitely while maintaining pluripotency. Leukemia inhibitory factor (LIF) can maintain self-renewal of mouse ES cells through activation of Stat3. However, LIF/Stat3 is dispensable for maintenance of

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