44535

Sigma-Aldrich

Atto Rho13 maleimide

Quality Level

fluorescence

λex 600 nm; λem 625 nm

storage temp.

−20°C

Application

Atto Rho13 maleimide is a new fluorescent label belonging to the class of rhodamines. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. ATTO Rho13 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

Melanie Kucki et al.
Journal of the Royal Society, Interface, 9(69), 727-733 (2011-08-26)
The incorporation of rhodamine dyes in the cell wall of diatoms Coscinodiscus granii and Coscinodiscus wailesii for the production of luminescent hybrid nanostructures is investigated. By systematic variation of the substitution pattern of the rhodamine core, we found that carbonic...
S R Kwon et al.
International journal of cosmetic science, 34(1), 97-101 (2011-09-29)
Enamel and dentin are susceptible to extrinsic and intrinsic stains. The purposes of this study were to determine the penetration pattern of Rhodamine B and dextran-conjugated Rhodamine B into the enamel and dentin as observed by confocal laser microscopy and...
Elizabeth S Childress et al.
Analytical chemistry, 84(3), 1235-1239 (2012-01-28)
We present dye-doped polymer nanoparticles that are able to detect mercury in aqueous solution at parts per billion levels via fluorescence resonance energy transfer (FRET). The nanoparticles are prepared by reprecipitation of highly fluorescent conjugated polymers in water and are...
Yasmine A Gomaa et al.
International journal of pharmaceutics, 438(1-2), 140-149 (2012-09-11)
Drug flux across microneedle (MN)-treated skin is influenced by the characteristics of the MN array, formed microconduits and physicochemical properties of the drug molecules in addition to the overall diffusional resistance of microconduits and viable tissue. Relative implication of these...

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