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  • 46043
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46043

Sigma-Aldrich

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

Synonym(s):
EtDi, EthD-1
Empirical Formula (Hill Notation):
C46H50Cl4N8
CAS Number:
Molecular Weight:
856.75
EC Number:
MDL number:
PubChem Substance ID:

Assay

~90% (HPCE)

Quality Level

form

solid

mp

≥250 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 493 nm in H2O
λex 528 nm; λem 617 nm in TE buffer; DNA

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

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Ethidium homodimer suitable for fluorescence, ~90% (HPCE)

Sigma-Aldrich

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Ethidium homodimer

Ethidium bromide BioReagent, for molecular biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

Ethidium bromide for biochemistry

Sigma-Aldrich

1.11615

Ethidium bromide

Ethidium bromide ~95% (HPLC)

Sigma-Aldrich

E8751

Ethidium bromide

assay

~90% (HPCE)

assay

-

assay

-

assay

~95% (HPLC)

form

solid

form

powder

form

solid

form

-

mp

≥250 °C (lit.)

mp

260-262 °C (dec.) (lit.)

mp

261-264 °C

mp

260-262 °C (dec.) (lit.)

solubility

DMF: soluble

solubility

-

solubility

40 g/L

solubility

-

fluorescence

λex 493 nm in H2O

fluorescence

λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 526 nm; λem 605 nm in aqueous buffer

fluorescence

-

fluorescence

-

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react 3

Storage Class Code

4.3 - Hazardous materials, which set free flammable gases upon contact with water

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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