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46393

Sigma-Aldrich

Fluo-3

suitable for fluorescence, ~70%

Synonym(s):
4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid
Empirical Formula (Hill Notation):
C36H30Cl2N2O13
CAS Number:
Molecular Weight:
769.54
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Assay

~70%

form

solid

solubility

H2O: soluble

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1

InChI

1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

Inchi Key

OZLGRUXZXMRXGP-UHFFFAOYSA-N

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Fluo-3 suitable for fluorescence, ~70%

Sigma-Aldrich

46393

Fluo-3

Fluo 3-AM ≥90% (TLC)

Sigma-Aldrich

73881

Fluo 3-AM

FLUO 3/AM Membrane-permeable version of FLUO 3. Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.

Sigma-Aldrich

343242-M

FLUO 3/AM

Fluoranthene 98%

Sigma-Aldrich

F807

Fluoranthene

assay

~70%

assay

≥90% (TLC)

assay

≥95% (HPLC)

assay

98%

form

solid

form

powder

form

solid

form

-

solubility

H2O: soluble

solubility

-

solubility

DMSO: 5 mM, DMF: soluble

solubility

-

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

fluorescence

λex 506 nm; λem 525 nm in 0.1 M Tris pH 8.0, esterase

fluorescence

-

fluorescence

-

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Application

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding.

Other Notes

Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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