This product is not tested for carbon dioxide in a supercritical state, so no information is available.
46955
Fluorescein
suitable for fluorescence, free acid
Synonym(s):
Acid Yellow 73
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$161.00
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$725.00
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$1,785.00
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About This Item
Empirical Formula (Hill Notation):
C20H12O5
CAS Number:
Molecular Weight:
332.31
EC Number:
219-031-8
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Colour Index Number:
45350
Beilstein/REAXYS Number:
94324
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InChI key
GNBHRKFJIUUOQI-UHFFFAOYSA-N
InChI
1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
Quality Level
Gene Information
human ... PTPN1(5770), PTPRF(5792)
assay
>95.0% (HPLC)
form
powder or crystals
mol wt
332.31 g/mol
concentration
≤100% (2-(6-hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid)
technique(s)
titration: suitable
color
red
mp
320 °C
solubility
methanol: 0.1 g/10 mL, orange to very deep orange
λmax
486.0-501.0 nm
fluorescence
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
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Related Categories
1 of 4
This Item | Y0000796 | 1277004 | 46960 |
|---|---|---|---|
| grade for fluorescence | grade pharmaceutical primary standard | grade pharmaceutical primary standard | grade - |
| suitability suitable for fluorescence | suitability - | suitability - | suitability suitable for fluorescence |
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) titration: suitable |
| fluorescence λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0 | fluorescence - | fluorescence - | fluorescence λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0 |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 200 |
| Gene Information human ... PTPN1(5770), PTPRF(5792) | Gene Information - | Gene Information - | Gene Information - |
Application
Fluorescein has been used:
- in the permeability assay of Caco-2 cells[1][2]
- to test its suitability to serve as lifetime fluorescence standards[3]
- as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin[4]
- as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay[5]
General description
Fluorescein dye is the most commonly used fluorescent probe.[6] It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation.[7] Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.[8]
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hirofumi Shoda et al.
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Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Fluorescein, Quinine Sulfate and Green Fluorescent Protein
Kristoffersen AS, et al.
Journal of Fluorescence, 28(5), 1065-1073 (2018)
Diagnostic agents
Robert-Gnansia E
Drugs During Pregnancy and Lactation, 502-513 (2007)
Experimental recovery of intrinsic fluorescence and fluorophore concentration in the presence of hemoglobin: spectral effect of scattering and absorption on fluorescence
Du Le VN, et al.
Journal of Biomedical Optics, 20(12), 127003-127003 (2015)
Microfluidic gut-on-a-chip with three-dimensional villi structure
Shim KY, et al.
Biomedical Microdevices, 19(2), 37-37 (2017)
Articles
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
Fluorescence quenching microscopy visualizes 2D materials like graphene and MoS2 rapidly, inexpensively, and with high fidelity.
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Dear Sir or Madam, Could you please clarify - is it possible to use fluorescein for carbon dioxide in a supercritical state?
1 answer-
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What is a free acid?
1 answer-
A free acid is the acidic form of a substance in which all acidic groups are fully protonated.
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What is the size in kDa of this Fluorescein?
1 answer-
This product has a molecular weight size of 332.32 g/mol or 332.32 daltons. The kDa unit designates kilodaltons, 1 kDa is equivalent to 1000 daltons.
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How long can this product be kept once it's made into a solution? If it can be kept more than 1 day; what are the storage conditions? Thank you
1 answer-
The stability of this product is not determined. However, various sources state that this product is stable in solvent at -80°C for 6 months; at -20°C for 1 month (protect from light).
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What is Product 46955, Fluorescein, soluble in?
1 answer-
According to the chemicals encyclopedia published by the Royal Society of Chemistry : 13th Edition, Fluorescein is soluble in hot alcohol and glacial acetic acid. Also, it is soluble in alkali hydroxides or carbonates. This product is insoluble in water, benzene, chloroform and ether.
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How should Product 46955, Fluorescein, be stored?
1 answer-
We recommend storing this product at room temperature.
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What is the dye content of Product 46955, Fluorescein?
1 answer-
According to our supplier, the dye content of pure Fluorescein is a minimum of 90%. We do not test the purity of this product since it is not one of our specifications.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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