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46960

Sigma-Aldrich

Fluorescein sodium salt

BioReagent, suitable for fluorescence

Synonym(s):

Acid Yellow 73, NaFl, Sodium fluorescein, Yellow No. 8, D&C;Yellow No. 8, NaFluo, Uranine

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$19.10
100 G
$49.40
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$153.00

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25 G
$19.10
100 G
$49.40
500 G
$153.00

About This Item

Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
Molecular Weight:
376.27
Colour Index Number:
45350
Beilstein/REAXYS Number:
3833041
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32

$19.10


In StockDetails


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product line

BioReagent

Quality Level

form

powder

technique(s)

titration: suitable

fluorescence

λex 460 nm; λem 515 nm(lit.)
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

InChI key

RGPLGPBQJOQFJS-UHFFFAOYSA-L

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General description

Fluorescein Sodium Salt (FSS) is a xanthene dye having a polycrystalline structure with a monoclinic system. Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol.

Application

Fluorescein Sodium Salt (FSS) is used to conduct in-vivo skin studies.[1]

Xanthene fluorophores, like Fluorescein Sodium Salt (FSS), are versatile compounds used across diverse fields of chemistry and life sciences.[2]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

423.7 °F - Pensky-Martens closed cup

flash_point_c

217.6 °C - Pensky-Martens closed cup

ppe

Eyeshields, Gloves, type N95 (US)


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Joshua L Turnbull et al.
Journal of the American Chemical Society, 143(16), 6194-6201 (2021-04-03)
Xanthene fluorophores, like fluorescein, have been versatile molecules across diverse fields of chemistry and life sciences. Despite the ubiquity of 3-carboxy and 3-sulfonofluorescein for the last 150 years, to date, no reports of 3-phosphonofluorescein exist. Here, we report the synthesis
Sara Seabrooke et al.
American journal of physiology. Cell physiology, 305(5), C558-C567 (2013-06-28)
The blood-brain barrier (BBB) physiologically isolates the brain from the blood and, thus, plays a vital role in brain homeostasis. Ion transporters play a critical role in this process by effectively regulating access of chemicals to the brain. Organic anion-transporting
Christopher Price et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 26(2), 277-285 (2010-08-18)
Since proposed by Piekarski and Munro in 1977, load-induced fluid flow through the bone lacunar-canalicular system (LCS) has been accepted as critical for bone metabolism, mechanotransduction, and adaptation. However, direct unequivocal observation and quantification of load-induced fluid and solute convection
Ken-ichiro O'goshi et al.
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI), 12(3), 155-161 (2006-07-11)
Epicutaneous labeling or intradermal injection of the fluorescent sodium fluorescein is being used increasingly to investigate skin conditions in vivo when using non-invasive devices such as confocal scanning laser microscopy. Sodium fluorescein was used intravenously for decades for the examination
T H B Eriksen et al.
Journal of biomedical nanotechnology, 9(3), 492-498 (2013-04-30)
Electrospinning is a very versatile technique, which holds great potentials for several clinical applications. The ability to produce biocompatible nanofibres mimicking the extracellular matrix of the body in combination with both the large surface area and the adsorption/release properties of

Questions

  1. For Fluorescein sodium salt 46960, is there a fluorescence value for a positive PCR sample, and how would this value translate as a concentration of this powder?

    1 answer
    1. Sybr Green and Fluorescein sodium salt are both green dyes, and although their spectral ranges are close, it is important to consider their specific uses and characteristics. While Sybr Green is a popular dye for molecular use, Fluorescein sodium salt is a very economical product and may be useful for specific applications. However, there is no information comparing the two dyes for use in PCR.

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