47671

Sigma-Aldrich

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein/REAXYS Number:
505995
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

BioUltra

assay

≥99.5% (T)

form

liquid

autoignition temp.

932 °F

application(s)

hybridization: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.1%

refractive index

n20/D 1.447 (lit.)
n20/D 1.447

pH

7-9 (25 °C, 1 M in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

solubility

H2O: 10 M at 20 °C, clear, colorless

density

1.134 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES string

NC=O

λ

10 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

hazcat

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the best way to deionize Formamide, Product 47671?

    Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.

  6. Are aqueous solutions of Formamide, Product 47671, stable?

    In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).

  7. What is the molarity of the neat liquid Formamide, Product 47671?

    Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
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Protocols
GC Analysis of Class 2 Residual Solvents on OVI-G43
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