5,6-Diaminofluorescein diacetate

BioReagent, suitable for fluorescence, 98% (HPCE)

4,5-Diaminofluorescein diacetate, DAF-2 DA
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:

Quality Level

product line



98% (HPCE)


λex 491 nm; λem 513 nm


suitable for fluorescence

SMILES string




InChI key



5,6-Diaminofluorescein diacetate (DAF-2 DA), a cell permeable analog of DAF-2, is used in intracellular fluorimetric detection of nitric oxide/NOS production.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Andréa C Celotto et al.
European journal of pharmacology, 656(1-3), 88-93 (2011-02-09)
We investigated the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The relaxation response to HCl-induced extracellular acidification (7.4 to 6.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M) or KCl (45mM). The vascular...
H Kojima et al.
Neuroreport, 9(15), 3345-3348 (1998-12-17)
The biological functions of nitric oxide in the neuronal system remain controversial. Using a novel fluorescence indicator, DAF-2 DA, for direct detection of NO, we examined both acute rat brain slices and organotypic culture of brain slices to ascertain NO...
Shoji Tsuji et al.
Journal of pediatric hematology/oncology, 34(7), 500-502 (2012-09-01)
Chronic granulomatous disease (CGD) is an inherited disorder in which phagocytic leukocytes fail to generate superoxide (O(2)(-)) and antimicrobial oxidants. Patients with CGD develop recurrent and often life-threatening infections due to catalase-positive microorganisms. We attempted to measure the production of...
Juan L Turrion-Gomez et al.
Molecular plant pathology, 12(6), 606-616 (2011-07-05)
Nitric oxide (NO) production by Botrytis cinerea and the effect of externally supplied NO were studied during saprophytic growth and plant infection. Fluorescence analysis with 4,5-diaminofluorescein diacetate and electrochemical studies were conducted in vitro between 4 and 20 h of...
A C Celotto et al.
Nitric oxide : biology and chemistry, 23(4), 269-274 (2010-08-05)
To investigate the mechanism through which the extracellular alkalinization promotes relaxation in rat thoracic aorta. The relaxation response to NaOH-induced extracellular alkalinization (7.4-8.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M). The vascular reactivity experiments were performed...

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