50277

Sigma-Aldrich

5,6-Diaminofluorescein diacetate

BioReagent, suitable for fluorescence, 98% (HPCE)

Synonym(s):
4,5-Diaminofluorescein diacetate, DAF-2 DA
Empirical Formula (Hill Notation):
C24H18N2O7
CAS Number:
Molecular Weight:
446.41
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

product line

BioReagent

assay

98% (HPCE)

fluorescence

λex 491 nm; λem 513 nm

suitability

suitable for fluorescence

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(N)c(N)cc45)c1

InChI

1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChI key

PTSUYDXEEKDBQU-UHFFFAOYSA-N

Application

5,6-Diaminofluorescein diacetate (DAF-2 DA), a cell permeable analog of DAF-2, is used in intracellular fluorimetric detection of nitric oxide/NOS production.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Andréa C Celotto et al.
European journal of pharmacology, 656(1-3), 88-93 (2011-02-09)
We investigated the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The relaxation response to HCl-induced extracellular acidification (7.4 to 6.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M) or KCl (45mM). The vascular...
H Kojima et al.
Neuroreport, 9(15), 3345-3348 (1998-12-17)
The biological functions of nitric oxide in the neuronal system remain controversial. Using a novel fluorescence indicator, DAF-2 DA, for direct detection of NO, we examined both acute rat brain slices and organotypic culture of brain slices to ascertain NO...
Shoji Tsuji et al.
Journal of pediatric hematology/oncology, 34(7), 500-502 (2012-09-01)
Chronic granulomatous disease (CGD) is an inherited disorder in which phagocytic leukocytes fail to generate superoxide (O(2)(-)) and antimicrobial oxidants. Patients with CGD develop recurrent and often life-threatening infections due to catalase-positive microorganisms. We attempted to measure the production of...
Juan L Turrion-Gomez et al.
Molecular plant pathology, 12(6), 606-616 (2011-07-05)
Nitric oxide (NO) production by Botrytis cinerea and the effect of externally supplied NO were studied during saprophytic growth and plant infection. Fluorescence analysis with 4,5-diaminofluorescein diacetate and electrochemical studies were conducted in vitro between 4 and 20 h of...
A C Celotto et al.
Nitric oxide : biology and chemistry, 23(4), 269-274 (2010-08-05)
To investigate the mechanism through which the extracellular alkalinization promotes relaxation in rat thoracic aorta. The relaxation response to NaOH-induced extracellular alkalinization (7.4-8.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M). The vascular reactivity experiments were performed...

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