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50611

Sigma-Aldrich

Atto 700 maleimide

BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)

MDL number:
NACRES:
NA.32

product line

BioReagent

Quality Level

assay

≥90% (coupling to thiols)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 645 nm; λem 715 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 700 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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