50712

Sigma-Aldrich

Atto 465

BioReagent, suitable for fluorescence

MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

product line

BioReagent

fluorescence

λex 453 nm; λem 508 nm

suitability

in accordance for fluorescence
suitable for fluorescence

storage temp.

−20°C

SMILES string

OCl(=O)(=O)=O.Nc1ccc2C=C3C=CC(=N)C=C3N(CCCC(O)=O)c2c1

InChI

1S/C17H17N3O2.ClHO4/c18-13-5-3-11-8-12-4-6-14(19)10-16(12)20(15(11)9-13)7-1-2-17(21)22;2-1(3,4)5/h3-6,8-10,18H,1-2,7,19H2,(H,21,22);(H,2,3,4,5)

InChI key

WNDQPKGVFUAMOZ-UHFFFAOYSA-N

General description

Atto 465 is a new label with strong absorption and fluorescence and a large stoke′s shift (excitation maximum 453 nm, emission maximum 508 nm). The dye is characterized by high photostability and thermal stability. The dye is moderately hydrophilic, the NHS-ester and maleimide derivatives are very well soluble in polar solvents as DMSO, DMF or acetonitrile. At low temperatures or embedded into polymeric matrices it shows intense phosphorescence.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 465 is suitable for preparation of fluorescence labeling reagents and studies of the physicochemical properties of the dye.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Jutta Arden-Jacob et al.
Physical chemistry chemical physics : PCCP, 15(6), 1844-1853 (2012-12-19)
The excited state dynamics of the dye ATTO 465, a well-known fluorescence marker for biological applications, have been characterized in various solvents including THF, ethanol, methanol, water and the highly polar protic ionic liquid 2-hydroxyethylammonium formate (2-OH-EAF) by combining results...
Radek Macháň et al.
Analytical and bioanalytical chemistry, 399(10), 3547-3554 (2011-02-05)
Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion...
David J Posson et al.
Nature, 436(7052), 848-851 (2005-08-12)
Voltage-gated ion channels open and close in response to voltage changes across electrically excitable cell membranes. Voltage-gated potassium (Kv) channels are homotetramers with each subunit constructed from six transmembrane segments, S1-S6 (ref. 2). The voltage-sensing domain (segments S1-S4) contains charged...
Andriy Chmyrov et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 7(11), 1378-1385 (2008-10-30)
Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus...
Gerald Donnert et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(31), 11440-11445 (2006-07-26)
We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has been enabled by the elimination of molecular triplet state excitation as a...

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