5074

Sigma-Aldrich

PureCol EZ Gel solution

NACRES:
NA.75

Quality Level

biological source

bovine skin

sterility

sterile

assay

≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

form

solid

packaging

pkg of 35 mL

application(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

PureCol is used in cell culture, tissue engineering and as a coating material for medical devices.

Features and Benefits

PureCol EZ Gel is designed to improve gel consistency by providing a pre-formulated solution of media and collagen that have been adjusted to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. PureCol EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution. PureCol EZ Gel is available in 35 mL volume, and produced by aseptic processing. 3D gels allow for the study of the effects of the mechanical properties of the ECM, such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways. Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments. Furthermore, integrin-independent mechanical interactions resulting from the entanglement of matrix fibrils with cell extensions are possible in 3D systems, but not in 2D systems where the cells are attached to a flat surface.1-3

Other Notes

PureCol EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I bovine collagen at a concentration of approximately 5 mg/ml (0.5%), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.

Preparation Note

3-D Gel Preparation Procedure
1. Remove PureCol EZ Gel from 2-10°C storage. To prevent gelation, maintain temperature of product at 2-10°C.
2. Introduce PureCol EZ Gel into cell culture system. Cells can be added to the PureCol EZ Gel solution.
3. To form gel, warm to 37°C. The beginning of gelation will occur within 40 minutes, but allow approximately 90 to minutes for firm gel formation.

Legal Information

PureCol is a trademark of Advanced BioMatrix, Inc.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Tomas Castro-Dopico et al.
Cell reports, 32(1), 107857-107857 (2020-07-09)
Macrophages play a central role in intestinal immunity, but inappropriate macrophage activation is associated with inflammatory bowel disease (IBD). Here, we identify granulocyte-macrophage colony stimulating factor (GM-CSF) as a critical regulator of intestinal macrophage activation in patients with IBD and...
Muhammad H Zaman et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(29), 10889-10894 (2006-07-13)
Cell migration on 2D surfaces is governed by a balance between counteracting tractile and adhesion forces. Although biochemical factors such as adhesion receptor and ligand concentration and binding, signaling through cell adhesion complexes, and cytoskeletal structure assembly/disassembly have been studied...
Karen A Beningo et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(52), 18024-18029 (2004-12-17)
Fibroblasts in 2D cultures differ dramatically in behavior from those in the 3D environment of a multicellular organism. However, the basis of this disparity is unknown. A key difference is the spatial arrangement of anchored extracellular matrix (ECM) receptors to...
Hongmei Jiang et al.
Molecular biology of the cell, 16(11), 5070-5076 (2005-08-19)
Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips...
John Midkiff et al.
PloS one, 6(9), e25395-e25395 (2011-10-04)
The ability of the pathogenic yeast Candida albicans to interconvert between budded and hyphal growth states, herein termed the budded-to-hyphal transition (BHT), is important for C. albicans development and virulence. The BHT is under the control of multiple cell signaling...
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