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PureCol EZ Gel solution

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NACRES:
NA.75

biological source

bovine skin

Quality Level

sterility

sterile

Assay

≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

form

solid

packaging

pkg of 35 mL

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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This Item
C4243G1393CC300
Sigma-Aldrich

Sigma-Aldrich

G1393

Gelatin solution

sterility

sterile

sterility

sterile-filtered

sterility

sterile

sterility

-

assay

≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

assay

≥99.9% (SDS-PAGE)

assay

-

assay

-

form

solid

form

liquid

form

solution

form

solution

packaging

pkg of 35 mL

packaging

pkg of 100 mL, pkg of 20 mL

packaging

pkg of 100 mL, pkg of 20 mL

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

General description

PureCol is used in cell culture, tissue engineering and as a coating material for medical devices.

Features and Benefits

PureCol EZ Gel is designed to improve gel consistency by providing a pre-formulated solution of media and collagen that have been adjusted to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. PureCol EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution. PureCol EZ Gel is available in 35 mL volume, and produced by aseptic processing. 3D gels allow for the study of the effects of the mechanical properties of the ECM, such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways. Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments. Furthermore, integrin-independent mechanical interactions resulting from the entanglement of matrix fibrils with cell extensions are possible in 3D systems, but not in 2D systems where the cells are attached to a flat surface.1-3

Other Notes

PureCol EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I bovine collagen at a concentration of approximately 5 mg/ml (0.5%), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.

Preparation Note

3-D Gel Preparation Procedure
1. Remove PureCol EZ Gel from 2-10°C storage. To prevent gelation, maintain temperature of product at 2-10°C.
2. Introduce PureCol EZ Gel into cell culture system. Cells can be added to the PureCol EZ Gel solution.
3. To form gel, warm to 37°C. The beginning of gelation will occur within 40 minutes, but allow approximately 90 to minutes for firm gel formation.

Legal Information

PureCol is a trademark of Advanced BioMatrix, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Proceedings of the National Academy of Sciences of the United States of America, 101(52), 18024-18029 (2004-12-17)
Fibroblasts in 2D cultures differ dramatically in behavior from those in the 3D environment of a multicellular organism. However, the basis of this disparity is unknown. A key difference is the spatial arrangement of anchored extracellular matrix (ECM) receptors to
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Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips
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