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50842

Sigma-Aldrich

Atto 740-Biotin

suitable for fluorescence, ≥90.0% (HPCE)

NACRES:
NA.32

Quality Level

assay

≥90.0% (HPCE)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 740 nm; λem 764 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 740 belongs to a new generation of fluorescent labels for the near infrared spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. Atto 740 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
Atto 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction.
Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ-hybridization, flow cytometry and others, to identify streptavidin, avidin or extravidin-conjugates.

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Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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