Atto 740-Biotin

suitable for fluorescence, ≥90.0% (HPCE)


Quality Level


≥90.0% (HPCE)


λex 740 nm; λem 764 nm in 0.1 M phosphate pH 7.0


suitable for fluorescence

storage temp.


General description

Atto 740 belongs to a new generation of fluorescent labels for the near infrared spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. Atto 740 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
Atto 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction.
Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ-hybridization, flow cytometry and others, to identify streptavidin, avidin or extravidin-conjugates.

find more information


Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment.
Frey, H.G., et al.
Applied Physics Letters, 94, 241116-241116 (2009)
E Shane Price et al.
The journal of physical chemistry. B, 115(29), 9320-9326 (2011-06-22)
Fluorescence correlation spectroscopy (FCS) can be coupled with Förster resonance energy transfer (FRET) to detect intramolecular dynamics of proteins on the microsecond time scale. Here we describe application of FRET-FCS to detect fluctuations within the N-terminal and C-terminal domains of...
Scott B Raymond et al.
Journal of biomedical optics, 15(4), 046011-046011 (2010-08-31)
Near-infrared (NIR) fluorescence tomography of multiple fluorophores has previously been limited by the bandwidth of the NIR spectral regime and the broad emission spectra of most NIR fluorophores. We describe in vivo tomography of three spectrally overlapping fluorophores using fluorescence...
Zhiliang Cheng et al.
Bioconjugate chemistry, 22(10), 2021-2029 (2011-09-09)
Block copolymer-based vesicles have recently garnered a great deal of interest as nanoplatforms for drug delivery and molecular imaging applications due to their unique structural properties. These nanovesicles have been shown to direct their cargo to disease sites either through...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.