If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
51280
Hemin
BioXtra, from Porcine, ≥96.0% (HPLC)
Synonym(s):
Chloro(protoporphyrinato)iron(III), Chlorohemin, Chloroprotoporphyrin IX iron(III), Ferriprotoporphyrin IX chloride, Hemin(chloride)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 g | Available to ship TODAYfromMILWAUKEE | $83.70 | |
| 5 g | Available to ship TODAYfromMILWAUKEE | $307.00 | |
| 25 g | Available to ship TODAYfromMILWAUKEE | $1,110.00 |
About This Item
biological source
Porcine
Quality Level
product line
BioXtra
assay
≥96.0% (HPLC)
form
powder or crystals
technique(s)
tissue culture: suitable, transfection: suitable
storage temp.
2-8°C
SMILES string
Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C
InChI
1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;
InChI key
BTIJJDXEELBZFS-HXFTUNQESA-K
Application
- To study bacterial strains and growth conditions[1]
- Used in short-term colony assays (the culture medium consists of hemin along with other components)[2]
- Cell transfections[3]
Biochem/physiol Actions
Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) tissue culture: suitable, transfection: suitable | technique(s) toxicology assay: suitable | technique(s) - | technique(s) - |
| assay ≥96.0% (HPLC) | assay ≥90% | assay - | assay - |
| biological source Porcine | biological source bovine | biological source - | biological source - |
| form powder or crystals | form powder | form solid | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 51280-25G | 04061835541454 |
| 51280-5G | 04061832492391 |
| 51280-1G | 04061832492384 |
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the solubility of Hemin, product catalog # 51280, CAS 16009-13-5?
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The product with code 51280 can be dissolved in DMSO at a concentration of 1 mM. It is also soluble in 1.4 M ammonium hydroxide at a concentration of 50 mg/ml, and this can be achieved by heating the solution at 95°C for 5 minutes, resulting in a dark brown solution. Additionally, a stock solution of 0.25% hemin in 50% triethanolamine can be prepared. CAS number 16009-13-5, which corresponds to product 51280, is soluble in 1.4 M NaOH at a concentration of 25 mg/mL, and it is practically insoluble in water.
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How can hemin be dissolved for cell culture experiments in macrophages? Is it necessary to couple it to serum/BSA?
1 answer-
This product has not been tested for use in cell culture. However, a previously deleted product, H1652, has been tested. The H1652 was dissolved at 10 mg/mL in 1 M NaOH and added then added to the culture medium at 7 mg/L. This information would need to be validated for product 51280.
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How stable are stock solutions of hemin (dissolved in 1N NaOH and diluted to 1 mM with dH2O)? Can they be stored at 4C or -20C?
1 answer-
The solution stability has not been determined.
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What is the solubility of Product 51280, Hemin?
1 answer-
Hemin can be dissolved in DMSO at 1 mM. This product should be soluble in 1.4 M ammonium hydroxide (50 mg/ml) with 5 minutes of heating at 95 °C, yielding a dark brown solution. A stock solution of 0.25% hemin in 50% triethanolamine can be made.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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