Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro . MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle′s Balanced Salts, which make them suitable for use in atmospheres charged with CO2.
International journal of molecular sciences, 21(11) (2020-06-14)
This research aimed to assess the impact of cisplatin, depending on the concentration and exposure time, on the expression pattern of leptin in an endometrial cancer cell line. Ishikawa endometrial cancer cell cultures were incubated with cisplatin, at concentrations of
World journal of gastroenterology, 15(44), 5533-5540 (2009-11-26)
To investigate the possible use of the multiple cytokine production modulator, Y-40138, as a novel immunotherapy in the rat nonalcoholic steatohepatitis (NASH) model. We allocated 6-wk-old male F344 rats to choline-supplemented, L-amino acid-defined (CSAA) diet (control group), CSAA diet +
Journal of the Association for Research in Otolaryngology : JARO, 10(2), 151-160 (2009-01-16)
Osteoclasts are the only cells capable of carrying out bone resorption and therefore are responsible for the osteolysis seen in infectious diseases such as chronic otitis media and infected cholesteatoma. Pseudomonas aeruginosa is the most common organism isolated from these
