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Minimum Essential Medium

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

MEM, Minimum Essential Medium Eagle

Quality Level


for research or for further manufacturing use






cell culture | mammalian: suitable
cell culture | stem cell: suitable


NaHCO3: 2200 mg/L
Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
L-glutamine: no

storage temp.


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General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.


Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A novel pulsed STED microscopy method using FastFLIM and the phasor plots.
Sun Y, et al.
Proceedings of SPIE (2017)
Involvement of microRNA-23b in TNF-?-reduced BMSC osteogenic differentiation via targeting runx2.
Deng L, et al.
Journal of Bone and Mineral Metabolism, 1-13 (2017)
Debora Soncini et al.
Blood advances, 4(18), 4312-4326 (2020-09-12)
Metabolic reprogramming is emerging as a cancer vulnerability that could be therapeutically exploitable using different approaches, including amino acid depletion for those tumors that rely on exogenous amino acids for their maintenance. ʟ-Asparaginase (ASNase) has contributed to a significant improvement...
Michael J Tuvim et al.
PloS one, 4(1), e4176-e4176 (2009-01-13)
Influenza pneumonia causes high mortality every year, and pandemic episodes kill millions of people. Influenza-related mortality has been variously ascribed to an ineffective host response that fails to limit viral replication, an excessive host inflammatory response that results in lung...
Dirk M Reiter et al.
PLoS pathogens, 7(8), e1002166-e1002166 (2011-08-11)
Many viruses attach to target cells by binding to cell-surface glycans. To gain a better understanding of strategies used by viruses to engage carbohydrate receptors, we determined the crystal structures of reovirus attachment protein σ1 in complex with α-2,3-sialyllactose, α-2,6-sialyllactose...

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