51412C

Minimum Essential Medium

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Minimum Essential Medium Eagle, MEM
• NACRES: NA.75

Properties

• description: for research or for further manufacturing use
• Quality Level: 500
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable; cell culture | stem cell: suitable
• components: L-glutamine: no; NaHCO₃: 2200 mg/L; phenol red: 10.62 mg/L; Earle’s salts (5% CO₂): yes
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO₂ gas.

Application

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves