51431

Sigma-Aldrich

Atto Rho11 maleimide

NACRES:
NA.32

Quality Level

fluorescence

λex 671 nm; λem 595 nm

storage temp.

−20°C

Application

Atto Rho11 maleimide is a new rhodamine dye featuring a functionality for coupling to bio-molecules such as DNA, RNA, proteins etc. The label shows very high fluorescence efficiency and exceptionally high photostability. The dye is moderately hydrophilic. ATTO Rho11 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Rhodamine-inspired far-red to near-infrared dyes and their application as fluorescence probes.
Sun YQ, Liu J, Lv X, Liu Y, Zhao Y, Guo W.
Angewandte Chemie (Weinheim an der Bergstrasse, Germany), 51, 763407636-763407636 (2012)
Jan Klohs et al.
Basic research in cardiology, 103(2), 144-151 (2008-03-08)
Light in the near-infrared (NIR) region between 700-900 nm can penetrate deep into living tissue, thereby offering a unique opportunity to use near-infrared fluorescence (NIRF) imaging techniques to detect and visualize fluorescent probes in-vivo. In the past few years, many...
Chunbai He et al.
Biomaterials, 33(33), 8569-8578 (2012-08-22)
Polymeric nanoparticles have been widely applied to oral delivery of protein drugs, however, few studies focused on the systematical elucidation of the size-dependent oral absorption mechanism with well-defined polymeric nanoparticles. Rhodamine B labeled carboxylated chitosan grafted nanoparticles (RhB-CCNP) with different...
Jianghong Rao et al.
Current opinion in biotechnology, 18(1), 17-25 (2007-01-20)
In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including...

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