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52595

Sigma-Aldrich

Atto 514

suitable for fluorescence

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Synonym(s):
Atto 514 carboxy, Atto 514 free acid

Assay

≥90.0% (HPLC)

form

solid

mol wt

Mw 868 g/mol

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
514 nm

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

λ

(in water/methanol 1:1 with 0.1% perchloric acid)

UV absorption

λ: 511-517 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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52595

Atto 514

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67455

Atto 514 NHS ester

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93728

Atto 514 amine

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68606

Atto 594-Streptavidin

form

solid

form

solid

form

solid

form

solid

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

fluorescence

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

General description

Atto 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield and exceptional thermal and photo-stability. Thus Atto 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of the Argon-Ion laser.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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