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53048

5-Carboxy-tetramethylrhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

Synonym(s):

"4-Carboxytetramethylrhodamine N-succinimidyl ester", 5-TAMRA

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$182.00

5 MG

$616.00

$182.00

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About This Item

Empirical Formula (Hill Notation):
C29H25N3O7
CAS Number:
150810-68-7
Molecular Weight:
527.52
MDL number:
MFCD03453223
UNSPSC Code:
12352116
PubChem Substance ID:
57651396
NACRES:
NA.32

Key Documents

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product line

BioReagent

Quality Level

100

solubility

DMF: soluble
acetonitrile: soluble

fluorescence

λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4C([O-])=O)C(=O)ON5C(=O)CCC5=O)=[N+](\C)C)c1

InChI

1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)19-8-5-16(13-22(19)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3

Inchi Key

VWFRSNKRTNUMET-UHFFFAOYSA-N

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This Item
8.510308.51026C2734
5-Carboxy-tetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence

Sigma-Aldrich

53048

5-Carboxy-tetramethylrhodamine N-succinimidyl ester

5(6)-Carboxytetramethylrhodamine Novabiochem®

Sigma-Aldrich

8.51030

5(6)-Carboxytetramethylrhodamine

5-Carboxy-tetramethylrhodamine Novabiochem®

Sigma-Aldrich

8.51026

5-Carboxy-tetramethylrhodamine

5-Carboxytetramethylrhodamine

Sigma-Aldrich

C2734

5-Carboxytetramethylrhodamine

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

fluorescence

λex ~543 nm; λem ~570 nm in methanol

solubility

DMF: soluble, acetonitrile: soluble

solubility

-

solubility

-

solubility

water: slightly soluble

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

product line

BioReagent

product line

Novabiochem®

product line

Novabiochem®

product line

-

Application

5-Carboxy-tetramethylrhodamine N-succinimidyl ester (5-TAMRA) is used as an amine coupling reagent to form 5-carboxy-tetramethylrhodamine (5-TAMRA) derivatized compounds. 5-TAMRA derivatized compounds may be used in applications as enzyme substrates, molecular probes, molecular beacons and as fluorescence resonance energy transfer (FRET) partners.

Other Notes

Fluorescent labeling of DNA and RNA[1]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM9384
BCCM4456
BCCL2481
BCCH4156
BCCF8567

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Elke Duchardt-Ferner et al.
Nucleic acids research, 48(2), 949-961 (2019-11-23)
RNA aptamers-artificially created RNAs with high affinity and selectivity for their target ligand generated from random sequence pools-are versatile tools in the fields of biotechnology and medicine. On a more fundamental level, they also further our general understanding of RNA-ligand
Simona Allocca et al.
International journal of molecular sciences, 19(7) (2018-06-30)
The H1069Q substitution is the most frequent mutation of the Cu transporter ATP7B that causes Wilson disease in the Caucasian population. ATP7B localizes to the Golgi complex in hepatocytes, but, in the presence of excessive Cu, it relocates to the
D Proudnikov et al.
Nucleic acids research, 24(22), 4535-4542 (1996-11-15)
Several procedures have been described for fluorescent labeling of DNA and RNA. They are based on the introduction of aldehyde groups by partial depurination of DNA or oxidation of the 3'-terminal ribonucleoside in RNA by sodium periodate. Fluorescent labels with
Deenan Santhiya et al.
The journal of physical chemistry. B, 114(22), 7602-7608 (2010-05-18)
Possible interaction mechanisms between oligonucleotide (DNA) of 14 base pairs with cetyl trimethyl ammonium bromide (CTAB) were postulated based on fluorescence and fluorescence resonance energy transfer (FRET) studies. Detailed FRET investigations were carried out by fluorometric titrations of the surfactant
Lukas Grätz et al.
Scientific reports, 10(1), 13288-13288 (2020-08-09)
Fluorescence/luminescence-based techniques play an increasingly important role in the development of test systems for the characterization of future drug candidates, especially in terms of receptor binding in the field of G protein-coupled receptors (GPCRs). In this article, we present the
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