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Although the excitation and emission wavelengths are measured during quality control testing, there is no testing to determine the extinction coefficient for 53608 (Biotin (5-fluorescein) conjugate). There was no information on file regarding the extinction coefficient for product 53608.
53608
Biotin (5-fluorescein) conjugate
BioReagent, suitable for fluorescence, ≥90% (HPCE)
Synonym(s):
N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide
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About This Item
Empirical Formula (Hill Notation):
C33H32N4O8S
CAS Number:
Molecular Weight:
644.69
MDL number:
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.32
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product line
BioReagent
assay
≥90% (HPCE)
solubility
DMSO: soluble
fluorescence
λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
[H][C@]12CS[C@@H](CCCCC(=O)NCCNC(=O)c3ccc(c(c3)C(O)=O)C4=C5C=CC(=O)C=C5Oc6cc(O)ccc46)[C@@]1([H])NC(=O)N2
InChI
1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1
InChI key
DFUFXKZUEOKPSD-LFERIPGTSA-N
1 of 4
This Item | 46935 | 88596 | 21884 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0 | fluorescence λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0 | fluorescence λex 455 nm; λem 514 nm in 0.1 M Tris pH 8.0 | fluorescence λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase) |
| solubility DMSO: soluble | solubility DMF: soluble, DMSO: soluble | solubility - | solubility DMF: soluble, DMSO: soluble, methanol: soluble |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. - | storage temp. −20°C |
| assay ≥90% (HPCE) | assay ≥90% (HPCE) | assay ≥90% (HPCE) | assay ≥85% (HPCE) |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Application
Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Biotin-fluorescein conjugate for the fluorometric, accurate measurement of avidin and streptavidin[1].
Often referred to as Biotin (4-fluorescein) conjugate.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Microglia respond in a stereotypical pattern to a diverse array of pathological states. These changes are coupled to morphological and immunophenotypical alterations and the release of a variety of reactive species, trophic factors and cytokines that modify both microglia and
H J Gruber et al.
Biochimica et biophysica acta, 1381(2), 203-212 (1998-08-01)
A new fluorimetric assay is presented for the specific and reliable quantitation of >/=2 nM avidin and streptavidin. The assay is based on pronounced changes in the fluorescence properties of commercial fluorescein-biotin, or of a newly synthesized biotin-poly(ethylene glycol)-pyrene conjugate
G. Kada et al.
Biochimica et Biophysica Acta, 1427, 33-33 (1998)
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What is the extinction coefficient for product 53608.
1 answer-
Helpful?
-
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what are the solvents required to dilute these two reagents and also the recommended concentration?
1 answer-
The solubility of Biotin (5-fluorescein) conjugate 53608-1MG-F is not a specification for product release, but it should be soluble in DMSO at least up to a concentration of 3 mM.
Helpful?
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