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54702

Sigma-Aldrich

FITC–DEAE–Dextran

average mol wt 70,000

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Synonym(s):
FITC–DEAE–Dextran 70, Fluorescein isothiocyanate–Diethylaminoethyl–Dextran
NACRES:
NA.32

biological source

synthetic

conjugate

FITC conjugate

form

powder or crystals

mol wt

average mol wt 70,000

composition

nitrogen content, 2-5%

extent of labeling

0.001-0.020 mol/mol FITC glucose

technique(s)

permeability assay: suitable

fluorescence

λex 493 nm; λem 518 nm in 0.1 M Tris pH 8.0

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1 of 4

This Item
533796805953471
Sigma-Aldrich

Sigma-Aldrich

54702

FITC–DEAE–Dextran

Sigma-Aldrich

Sigma-Aldrich

53379

FITC-CM-Dextran

Sigma-Aldrich

Sigma-Aldrich

68059

FITC-CM-Dextran

Sigma-Aldrich

Sigma-Aldrich

53471

FITC-CM-Dextran

form

powder or crystals

form

powder or crystals

form

powder or crystals

form

powder or crystals

technique(s)

permeability assay: suitable

technique(s)

permeability assay: suitable

technique(s)

permeability assay: suitable

technique(s)

permeability assay: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

mol wt

average mol wt 70,000

mol wt

average mol wt 40,000

mol wt

average mol wt 4,000

mol wt

average mol wt 70,000

Application

FITC-DEAE-Dextran 70 is a cationic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-DEAE-Dextran 70 may be use to compare its selectivity versus neutral FITC-Dextran 70 or anionic FITC-CM-Dextran 70.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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J K Leypoldt et al.
Kidney international, 43(4), 837-844 (1993-04-01)
The influence of molecular charge on macromolecule transport during peritoneal dialysis was assessed by determining transperitoneal transport rates for fluorescent-labeled macromolecules (molecular radii from 15 to 40 A) that differed only in molecular charge: neutral dextran, anionic dextran sulfate and
Mary C Regier et al.
Scientific reports, 9(1), 6433-6433 (2019-04-25)
Concentration gradients of biochemical stimuli such as morphogens play a critical role in directing cell fate patterning across species and throughout development but are not commonly recapitulated in vitro. While in vitro biomolecule gradients have been generated using customized microfluidic
Carrier and dose effects on the pharmacokinetics of T-0128, a camptothecin analogue-carboxymethyl dextran conjugate, in non-tumor- and tumor-bearing rats.
Harada M, Murata J, Sakamura Y, et al.
J. Controlled Release, 71, 71-86 (2001)
Reduced diffusion of charge-modified, conformationally intact anionic Ficoll relative to neutral Ficoll across the rat glomerular filtration barrier in vivo.
Axelsson J, Sverrisson K, Rippe A, et al.
American Journal of Physiology: Renal Physiology, 301, F708-F712 (2011)
Takayuki Morikawa et al.
Scientific reports, 10(1), 6422-6422 (2020-04-15)
Physiological regulation of blood flow in bone marrow is important to maintain oxygen and glucose supplies but also the physiological hypoxic state of the hematopoietic stem cell (HSC) niche. However, regulatory mechanisms underlying microcirculation in the bone marrow (BM) niche

Articles

FITC-labelled polysaccharides

Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). This page discusses the impact of weight-average molecular weight on the respective molecule dimensions and other physical properties

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