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55156

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester

suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)

Synonym(s):

N-Succinimidyl 7-hydroxycoumarin-3-carboxylate, Umbelliferone-3-carboxylic acid N-succinimidyl ester

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$168.00

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$410.50

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About This Item

Empirical Formula (Hill Notation):
C14H9NO7
CAS Number:
134471-24-2
Molecular Weight:
303.22
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
57651516
MDL number:
MFCD00037568
Beilstein/REAXYS Number:
9419622
Assay:
≥95.0% (coupling assay to aminopropyl silica gel)

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Product Name

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester, suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)

InChI

1S/C14H9NO7/c16-8-2-1-7-5-9(13(19)21-10(7)6-8)14(20)22-15-11(17)3-4-12(15)18/h1-2,5-6,16H,3-4H2

SMILES string

Oc1ccc2C=C(C(=O)ON3C(=O)CCC3=O)C(=O)Oc2c1

InChI key

KFEBWCYYRFZMTJ-UHFFFAOYSA-N

assay

≥95.0% (coupling assay to aminopropyl silica gel)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 386 nm; λem 448 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)

Sigma-Aldrich

55156

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

Sigma-Aldrich

36801

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester

6-Maleimidohexanoic acid N-hydroxysuccinimide ester ≥98%, powder

Sigma-Aldrich

M9794

6-Maleimidohexanoic acid N-hydroxysuccinimide ester

5-Carboxy-fluorescein diacetate N-succinimidyl ester suitable for fluorescence, ≥95.0% (HPLC)

Sigma-Aldrich

87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

assay

≥95.0% (coupling assay to aminopropyl silica gel)

assay

≥96.0% (HPLC)

assay

≥98%

assay

≥95.0% (HPLC)

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

chloroform: 100 mg/mL, DMF: soluble (lit.)(lit.)

solubility

DMF: soluble, DMSO: soluble

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

fluorescence

λex 386 nm; λem 448 nm in 0.1 M Tris pH 9.0

fluorescence

λex 445 nm; λem 482 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

λex 492 nm; λem 517 nm

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

Application

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester (7-OHCCA-SE) is used as a reagent to conjugate 7-Hydroxycoumarin-3-carboxylic acid (7-OHCCA) to other molecules such as amino acids via amide chemistry.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK8960
070885/1
70885/1

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Y Oda et al.
Analytical biochemistry, 269(2), 230-235 (1999-05-01)
A fluorometric binding assay for lectin and yeast cells using the avidin-biotin system was previously reported (Y. Oda, M. Kinoshita, and K. Kakehi, Anal. Biochem. 254, 41-48, 1997). However, the true amount of bound lectin could not be determined by
R Cordfunke et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(13), 7396-7401 (1998-06-24)
The chromophore of photoactive yellow protein (PYP) (i.e., 4-hydroxycinnamic acid) has been replaced by an analogue with a triple bond, rather than a double bond (by using 4-hydroxyphenylpropiolic acid in the reconstitution, yielding hybrid I) and by a "locked" chromophore

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