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55222

Sigma-Aldrich

6-Carboxytetramethylrhodamine

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):
6-TAMRA
Empirical Formula (Hill Notation):
C25H22N2O5
CAS Number:
Molecular Weight:
430.45
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

description

single isomer

product line

BioReagent

assay

≥90% (HPLC)

form

solid

solubility

DMSO: soluble
methanol: soluble

fluorescence

λex 543 nm; λem 575 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4cc(ccc4C([O-])=O)C(O)=O)=[N+](\C)C)c1

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)20-11-14(24(28)29)5-8-17(20)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI key

COCMHKNAGZHBDZ-UHFFFAOYSA-N

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Application

6-Carboxytetramethylrhodamine (6-TAMRA) is used to create fluorescent DNA probes and labeled proteins/peptides. The corresponding succinimidyl ester is a single isomer fluorophore most commonly used in automated DNA sequencing.
The corresponding succinimidyl ester is a single isomer fluorophore most commonly used in automated DNA sequencing.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Certificate of Analysis

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Certificate of Origin

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