55607

Sigma-Aldrich

Atto 465 maleimide

BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)

MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

product line

BioReagent

assay

≥90% (coupling to thiols)

fluorescence

λex 452 nm; λem 502 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OCl(=O)(=O)=O.Nc1ccc2C=C3C=CC(=N)C=C3N(CCCC(=O)NCCN4C(=O)C=CC4=O)c2c1

InChI

1S/C23H23N5O3.ClHO4/c24-17-5-3-15-12-16-4-6-18(25)14-20(16)27(19(15)13-17)10-1-2-21(29)26-9-11-28-22(30)7-8-23(28)31;2-1(3,4)5/h3-8,12-14,24H,1-2,9-11,25H2,(H,26,29);(H,2,3,4,5)

InChI key

KHHWHZCVGMYUGL-UHFFFAOYSA-N

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 465 maleimide is suitable for fluorescence labeling of thiol/SH containing molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Certificate of Analysis

Certificate of Origin

David J Posson et al.
Nature, 436(7052), 848-851 (2005-08-12)
Voltage-gated ion channels open and close in response to voltage changes across electrically excitable cell membranes. Voltage-gated potassium (Kv) channels are homotetramers with each subunit constructed from six transmembrane segments, S1-S6 (ref. 2). The voltage-sensing domain (segments S1-S4) contains charged...
Radek Macháň et al.
Analytical and bioanalytical chemistry, 399(10), 3547-3554 (2011-02-05)
Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion...
Andriy Chmyrov et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 7(11), 1378-1385 (2008-10-30)
Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus...
Thiol reactive probes and chemosensors.
Peng H, Chen W, Cheng Y, et al.
Sensors, 12, 15907-15946 (2012)
Gerald Donnert et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(31), 11440-11445 (2006-07-26)
We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has been enabled by the elimination of molecular triplet state excitation as a...

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