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MilliporeSigma

56312C

Medium 199 Modified

with Earle′s Balanced Salts, with 0.68 mM L-glutamine, without sodium bicarbonate, dry powder, suitable for cell culture

Synonym(s):

Medium 199

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.71

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description

for research or for further manufacturing use

Quality Level

500

form

dry powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: 100 mg/L
NaHCO3: no
Earle’s salts (5% CO2): yes
phenol red: 21.42 mg/L

shipped in

ambient

storage temp.

2-8°C

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This Item
M252051322CM3769
SAFC

SAFC

56312C

Medium 199 Modified

Sigma-Aldrich

Sigma-Aldrich

M2520

Medium 199

SAFC

SAFC

51322C

Medium 199 Modified

Sigma-Aldrich

Sigma-Aldrich

M3769

Medium 199

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

dry powder

form

powder

form

liquid

form

powder

components

L-glutamine: 100 mg/L
NaHCO3: no
Earle’s salts (5% CO2): yes
phenol red: 21.42 mg/L

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

components

L-glutamine: 100 mg/L
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
NaHCO3: 350 mg/L
phenol red: 21.24 mg/L

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

description

for research or for further manufacturing use

description

-

description

for research or for further manufacturing use

description

-

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.

Other Notes

Contains all L-form amino acids

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0650
RNBL5904
RNBJ1832
RNBJ0482
RNBH5060

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Wuping Li et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 17(12), 2067-2077 (2009-07-16)
Recombinant adeno-associated virus (AAV) vectors expressing the cystic fibrosis transmembrane conductance regulator (CFTR) gene have been used to deliver CFTR to the airway epithelium of cystic fibrosis (CF) patients. However, no significant CFTR function has been demonstrated likely due to

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