Stock solutions of Calcein-AM in DMSO are stable at -20 deg. C for up to 12 months when protected from light. Upon dilution to an aqueous working solution (DPBS) it should be used the same day.
56496
Calcein-AM
Small Package (20 X 50 μg ), ≥90.0% (HPLC)
Synonym(s):
Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, fluorescein complexone, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
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About This Item
Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
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InChI key
XKFSBWQWNMZWFA-UHFFFAOYSA-N
SMILES string
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
InChI
1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
assay
≥90.0% (HPLC)
fluorescence
λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | C1359 | 206700 | 17783 |
|---|---|---|---|
| fluorescence λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ | fluorescence - | fluorescence λex 494 nm, λem 517 nm | fluorescence λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| assay ≥95.0% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) |
Application
Calcein-AM is used as a cell viability stain and as a neutral substrate for multidrug (MDR) efflux transporters. In cancer research, it is used as a neutral substrate for multidrug resistance protein MRP. Fluorescence assays to determine human erythrocyte viability and aging uses Calcein-AM as a probe.[1]
Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.
General description
Calcein-AM, also known as Calcein-acetoxymethyl ester, is a non-fluorescent molecule converted into an anionic fluorescent form by intracellular esterase enzymes. It provides a continuous visualization of adherent cells during the experiment.[2] Calcein-AM is a vital dye that introduces Calcein in living cells with intact cell membranes. The fluorescent part of Calcein-AM localizes in the intracellular spaces after esterase-dependent cellular trapping, displaying cytotoxic activity in cells.[3]
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Daniela Bratosin et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 66(1), 78-84 (2005-05-26)
A highly sensitive, fast, and simple flow cytometric assay to assess human red blood cell (RBCs) viability and aging is reported. The assay described in this report is based on the use of acetoxymethyl ester of calcein (calcein-AM), a fluorescein
Jacopo Uggeri et al.
Histochemistry and cell biology, 122(5), 499-505 (2004-10-27)
Calcein-acetoxymethylester (calcein-AM) is a non-fluorescent, cell permeant compound, which is converted by intracellular esterases into calcein, an anionic fluorescent form. It is used in microscopy and fluorometry and provides both morphological and functional information of viable cells. In this study
B Jonsson et al.
European journal of cancer (Oxford, England : 1990), 32A(5), 883-887 (1996-05-01)
The aim of this study was to determine the in vitro cytotoxicity of calcein acetoxymethyl ester (Calcein/AM) on primary cultures derived from solid and haematological human tumours. Calcein/AM is a fluorescent dye that localises intracellularly after esterase-dependent cellular trapping and
Xiao-Cong Huang et al.
Biochemical pharmacology, 85(9), 1257-1268 (2013-02-19)
High intrinsic or acquired expression of membrane spanning, adenosine triphosphate binding cassette (ABC) transporter proteins, such as P-glycoprotein (P-gp), in cancers represents a major impediment to chemotherapy, with accelerated drug efflux leading to multi-drug resistance (MDR). Although ABC transporter inhibitors
Bao-Ling Du et al.
Journal of biomedical materials research. Part A, 103(4), 1533-1545 (2014-07-22)
Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs
Articles
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
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I am wondering how long is Calcein-AM stable in solution approximately ? We dilute Calcein-AM in DPBS 1X supplemented with 1 % HI-FBS. Can we prepare this solution of Calcein-AM diluted in DPBS 1X -1 % HI-FBS the morning to use it the afternoon ? Thanks
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