MilliporeSigma
All Photos(1)

Documents

56759

Sigma-Aldrich

Atto 425

BioReagent, suitable for fluorescence

Sign Into View Organizational & Contract Pricing

Empirical Formula (Hill Notation):
C22H27NO6
CAS Number:
Molecular Weight:
401.45
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥90% (HPLC)

form

(Report result)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, >90%
425 nm, >90%

fluorescence

λex 440 nm; λem 484 nm

λ

in PBS, pH 7.4
in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 423-429 nm Amax

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(O)=O)c3cc2OC1=O

InChI

1S/C22H27NO6/c1-5-28-20(26)16-10-14-9-15-13(2)12-22(3,4)23(8-6-7-19(24)25)17(15)11-18(14)29-21(16)27/h9-11,13H,5-8,12H2,1-4H3,(H,24,25)

InChI key

WNDDWSAHNYBXKY-UHFFFAOYSA-N

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
419865696370425
Atto 425 BioReagent, suitable for fluorescence

Sigma-Aldrich

56759

Atto 425

Sigma-Aldrich

Sigma-Aldrich

41986

Atto 550 azide

Atto Thio12 BioReagent, suitable for fluorescence

Sigma-Aldrich

56963

Atto Thio12

Atto 590 BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

70425

Atto 590

assay

≥90% (HPLC)

assay

>90% (HPLC)

assay

≥90% (HPLC)

assay

≥90% (HPLC)

form

(Report result)

form

-

form

solid

form

powder

fluorescence

λex 440 nm; λem 484 nm

fluorescence

λex 554 nm; λem 576 nm±10 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 is suitable for preparation of fluorescence labeling reagents.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Imidazole BioUltra, ≥99.5% (GC)

Sigma-Aldrich

56749

Imidazole

Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)
The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution.
Praus, P., et al.
Spectroscopy: An International Journal, 24, 303-307 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service