57000

Indigo carmine

suitable for microscopy

• Synonym(s): Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine
• Empirical Formula (Hill Notation): C₁₆H₈N₂Na₂O₈S₂
• CAS Number: 860-22-0
• Molecular Weight: 466.35
• Colour Index Number: 73015
• Beilstein/REAXYS Number: 4103904
• EC Number: 212-728-8
• MDL number: MFCD00005723
• UNSPSC Code: 12171500
• PubChem Substance ID: 57651616
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 100
• technique(s): microbe id | staining: suitable
• loss: ≤5% loss on drying
• pH: 3.87 (30 °C, 7.14 g/L)
• mp: >340 °C
• solubility: methanol: 0.01 g/10 mL, blue to very deep blue
• density: 0.71 g/cm³ at 29 °C
• εmax: ≥350 at 603-613 nm
• suitability: suitable for microscopy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O
• InChI: 1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;
• InChI key: KHLVKKOJDHCJMG-QDBORUFSSA-L

Description

Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae.[1] It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.[2]

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.[3]

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Gloves