57000

Sigma-Aldrich

Indigo carmine

for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

Synonym(s):
Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine, Acid Blue 74
Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

for microscopy (Bact., Hist.)
indicator (pH 11.5-14.0)

form

powder

application(s)

hematology: suitable
histology: suitable

loss

≤5% loss on drying

pH

3.87 (30 °C, 7.14 g/L)

mp

>340 °C

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

density

0.71 g/cm3 at 29 °C

εmax

≥350 at 603-613 nm

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

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Journal of environmental sciences (China), 24(3), 464-472 (2012-06-05)
A new method for the preparation of rubber sheet strewn with titanium dioxide particles (TiO2-strewn sheet) is presented. This simple and low cost method is based on the use of TiO2 powder (Degussa P25) being strewn onto the sheet made...
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Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society, 25 Suppl 1, 7-12 (2013-03-27)
The indication of endoscopic submucosal dissection for Barrett's esophageal adenocarcinoma (BEA) is superficial BEA without lymph node metastasis. The characteristic endoscopic findings of superficial BEA are elevation, depression, and color change. Indigocarmine spreading is useful for the diagnosis of lateral...

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