58030

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

• Synonym(s): 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT
• Empirical Formula (Hill Notation): C₁₉H₁₃ClIN₅O₂
• CAS Number: 146-68-9
• Molecular Weight: 505.70
• Beilstein: 4093224
• EC Number: 205-676-2
• MDL number: MFCD00011961
• PubChem Substance ID: 57651636
• NACRES: NA.25

Properties

• Quality Level: 200
• product line: BioReagent
• assay: ≥97.0% (calc. on dry substance, NT)
• mp: 240 °C (dec.) (lit.); ~245 °C (dec.)
• solubility: methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)
• application(s): histology
• SMILES string: [Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4
• InChI: 1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
• InChI key: JORABGDXCIBAFL-UHFFFAOYSA-M

Description

Application

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.

Electron acceptor for the colorimetric assay of various dehydrogenases

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H228 - H335
• Precautionary Statements: P210 - P240 - P241 - P261 - P271 - P280
• Hazard Classifications: Flam. Sol. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 4.1B - Flammable solid hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves