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58030

Sigma-Aldrich

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym(s):
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT
Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
Molecular Weight:
505.70
Beilstein:
4093224
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioReagent

assay

≥97.0% (calc. on dry substance, NT)

mp

240 °C (dec.) (lit.)
~245 °C (dec.)

solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

application(s)

histology

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

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Application

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Electron acceptor for the colorimetric assay of various dehydrogenases

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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