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MilliporeSigma

60615

Kanamycin sulfate

mixture of Kanamycin A (main component) and Kanamycin B and C

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5 G

$134.00

25 G

$537.00

$134.00

Available to ship onFebruary 10, 2026Details

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About This Item

Linear Formula:
C18H36-37N4-5O10-11 · H2SO4
CAS Number:
70560-51-9
UNSPSC Code:
51281654
NACRES:
NA.85
PubChem Substance ID:
57651778
MDL number:
MFCD00070253
Beilstein/REAXYS Number:
3874279

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InChI

1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1

SMILES string

OS(O)(=O)=O.NC[C@H]1O[C@@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI key

OOYGSFOGFJDDHP-KMCOLRRFSA-N

form

powder or crystals

potency

≥750 units per mg (calculated with reference to dried substance)

ign. residue

≤0.5% (as SO4)

loss

≤1.5% loss on drying

color

white to off-white

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma

mode of action

protein synthesis | interferes

Quality Level

200

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1 of 4

This Item
K4000B5264K1637
Kanamycin sulfate mixture of Kanamycin A (main component) and Kanamycin B and C

Sigma-Aldrich

60615

Kanamycin sulfate

Kanamycin sulfate from Streptomyces kanamyceticus Animal Component-free

Sigma-Aldrich

K4000

Kanamycin sulfate from Streptomyces kanamyceticus

Kanamycin B sulfate salt aminoglycoside antibiotic

Sigma-Aldrich

B5264

Kanamycin B sulfate salt

Kanamycin sulfate from Streptomyces kanamyceticus meets USP testing specifications, powder

Sigma-Aldrich

K1637

Kanamycin sulfate from Streptomyces kanamyceticus

antibiotic activity spectrum

Gram-negative bacteria, mycobacteria, Gram-positive bacteria, mycoplasma

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycoplasma

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder or crystals

form

powder

form

powder or crystals

form

powder

potency

≥750 units per mg (calculated with reference to dried substance)

potency

≥750 μg per mg (dry basis)

potency

-

potency

≥750 μg per mg

color

white to off-white

color

white to off-white

color

-

color

-

ign. residue

≤0.5% (as SO4)

ign. residue

-

ign. residue

-

ign. residue

-

General description

Chemical structure: aminoglycoside

Application

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium.†† Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL.

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial Spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Packaging

5g, 25 g

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Disclaimer

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000489254
0000489254
0000430587
0000430587
0000407499

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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