MilliporeSigma
All Photos(1)

61305

Sigma-Aldrich

Penicillinase from Bacillus cereus

lyophilized, powder, white, ~13 U/mg

Synonym(s):
Penicillin amido-β-lactam hydrolase, β-Lactamase II, Cephalosporinase, β-Lactamase I from Bacillus cereus
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Bacillus sp. (Bacillus cereus)

Quality Level

form

powder

quality

lyophilized

specific activity

~13 U/mg

mol wt

Mr ~30000

color

white

foreign activity

β-lactamase II ≤2%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.

Application

Used in the production of penicillin.
Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Other Notes

Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service