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61909

Sigma-Aldrich

Atto Rho14 NHS ester

BioReagent, suitable for fluorescence

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NACRES:
NA.32

product line

BioReagent

Assay

≥90.0% (HPLC)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

extent of labeling

≥90.0%

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 624.0-630.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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assay

≥90.0% (HPLC)

assay

90% (Coupling efficiency with benzylamine, HPLC)

assay

≥90.0% (HPLC)

assay

≥90.0% (HPLC)

form

solid

form

solid

form

powder

form

solid

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Application

Atto Rho14 NHS ester is a new rhodamine featuring a functionality for coupling to bio-molecules such as DNA, RNA or proteins. The label shows strong absorption and extraordinarily high fluorescence quantum yield. ATTO Rho14 is the brightest label available in this wavelength range. The dye exhibits an exceptionally high photostability. In common with most ATTO-labels, absorption and fluorescence are pH-independent in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic. After coupling to a substrate ATTO Rho14 carries a net electrical charge of +1.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Yuan-Qiang Sun et al.
Angewandte Chemie (International ed. in English), 51(31), 7634-7636 (2012-06-08)
Probes to dye for: Rhodamine-inspired Si-pyronine, Si-rhodamine, Te-rhodamine, and Changsha NIR dyes have been developed recently. These dyes show fluorescence in the far-red to near-infrared region, while retaining the advantages of the original rhodamines, such as high fluorescence quantum yield
Chao Ma et al.
Analytica chimica acta, 734, 69-78 (2012-06-19)
This article describes the design and preparation of a novel fluorescence resonance energy transfer (FRET)-based ratiometric sensor with the polymer nanoparticle as scaffold for detecting Hg(2+) in aqueous media. In this study, a fluorescent dye fluorescein isothiocyanate (FITC, served as
Raphael Alford et al.
Molecular imaging, 8(6), 341-354 (2009-12-17)
Fluorophores are potentially useful for in vivo cancer diagnosis. Using relatively inexpensive and portable equipment, optical imaging with fluorophores permits real-time detection of cancer. However, fluorophores can be toxic and must be investigated before they can be administered safely to
Jan Klohs et al.
Basic research in cardiology, 103(2), 144-151 (2008-03-08)
Light in the near-infrared (NIR) region between 700-900 nm can penetrate deep into living tissue, thereby offering a unique opportunity to use near-infrared fluorescence (NIRF) imaging techniques to detect and visualize fluorescent probes in-vivo. In the past few years, many
Chunbai He et al.
Biomaterials, 33(33), 8569-8578 (2012-08-22)
Polymeric nanoparticles have been widely applied to oral delivery of protein drugs, however, few studies focused on the systematical elucidation of the size-dependent oral absorption mechanism with well-defined polymeric nanoparticles. Rhodamine B labeled carboxylated chitosan grafted nanoparticles (RhB-CCNP) with different

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