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61911

Sigma-Aldrich

Atto 520 azide

suitable for fluorescence

assay

≥80.0% (HPCE)

Quality Level

form

solid

mol wt

Mw 681 g/mol

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 516 nm; λem 545 nm±10 nm in PBS, pH 7.4

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. Above pH 7.0 the dye exists in an equilibrium with a colourless form (pseudobase). This is a reversible reaction, which does not interfere with the process of coupling to proteins etc. On lowering the pH absorption and fluorescence are restored fully. For details of coupling see our recommended labeling procedures The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line.
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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