62285

Lipase from Aspergillus oryzae

lyophilized, powder, white, ~50 U/mg

• CAS Number: 9001-62-1
• EC Number: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: powder
• Quality Level: 100
• quality: lyophilized
• specific activity: ~50 U/mg
• color: white
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Lipase is a carboxylesterase, which belongs to the α/β hydrolase fold family. It consists of a lid domain, hinge domain, and a catalytic triad Ser-His-Asp/Glu. Aspergillus oryzae lipase has a three-dimensional structure is an α/β hydrolase fold similar to that of the esterase enzyme.[1]

Application

Lipase from Aspergillus oryzae has been used as a component of mixed enzyme solution to determine the viable cell numbers in the biofilms formed by Listeria monocytogenes strains.[2]

Biochem/physiol Actions

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.[3]

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.[4] Aspergillus oryzae lipase might be used in the production of biodiesel from waste cooking oil.[5] It is used in the acetylation of cellulose and ochratoxin A degradation, and asymmetric organic synthesis.[1] Lipase from Aspergillus oryzae is an industrial enzyme that has various uses in food, detergent, and pharmaceutical industries.[5][1] It is useful in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis.[6]

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314 as substrate)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)