Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)
Characterization; In the synthesis of fatty hydroxamic acids; Esterification