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62301

Sigma-Aldrich

Lipase from Aspergillus niger

powder (fine), ~200 U/g

Synonym(s):
Lipase AP6
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

form

powder (fine)

specific activity

~200 U/g

mol wt

Mr ~45000

storage temp.

2-8°C

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Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol acetic acid per minute at pH 7.4 and 40 °C (triacetin, Cat. No. 90240 as substrate) 50 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40 °C

Other Notes

Selective acylation and deacylation of furanose and pyranose derivatives; Enantioselective hydrolysis of 2-methyl 3-acetoxy esters; Resolution of the diols of bicycloheptane and bicyclooctane by enzymic hydrolysis; Glyceride synthesis

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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