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62310

Sigma-Aldrich

Lipase from Rhizopus niveus

powder (fine), ≥1.5 U/mg

Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.26

biological source

fungus (Rhizopus niveus)

Quality Level

form

powder (fine)

specific activity

≥1.5 U/mg

mol wt

Mr ~83000

storage temp.

2-8°C

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General description

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem/physiol Actions

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Other Notes

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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