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63493

Sigma-Aldrich

Hoechst 34580

Trihydrochloride salt, ≥98.0% (HPLC)

Synonym(s):
N,N-Dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]benzenamine trihydrochloride, HOE 34580
Empirical Formula (Hill Notation):
C27H29N7 · 3HCl
CAS Number:
Molecular Weight:
560.95
PubChem Substance ID:
NACRES:
NA.32

Quality Level

Assay

≥98.0% (HPLC)

quality

Trihydrochloride salt

solubility

H2O: 1 mg/mL, clear, slightly yellow to deep greenish-yellow

fluorescence

λex 357 nm; λem 490 nm±10 nm in H2O (free dye)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3nc([nH]c3c2)-c4ccc5nc([nH]c5c4)-c6ccc(cc6)N(C)C

InChI

1S/C27H29N7.3ClH/c1-32(2)20-7-4-18(5-8-20)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-9-21(17-25(23)31-27)34-14-12-33(3)13-15-34;;;/h4-11,16-17H,12-15H2,1-3H3,(H,28,30)(H,29,31);3*1H

InChI key

IPMZLKUUQSFUTA-UHFFFAOYSA-N

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This Item
94403H4415SML1446
Hoechst 34580 Trihydrochloride salt, ≥98.0% (HPLC)

Sigma-Aldrich

63493

Hoechst 34580

Hoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC)

Sigma-Aldrich

94403

Hoechst 33258 solution

HC-030031 ≥98% (HPLC), powder

Sigma-Aldrich

H4415

HC-030031

HTH-01-015 ≥98% (HPLC)

Sigma-Aldrich

SML1446

HTH-01-015

quality

Trihydrochloride salt

quality

-

quality

-

quality

-

solubility

H2O: 1 mg/mL, clear, slightly yellow to deep greenish-yellow

solubility

DMF: soluble, H2O: soluble

solubility

DMSO: 10 mg/mL, clear

solubility

DMSO: 5 mg/mL, clear (warmed)

fluorescence

λex 357 nm; λem 490 nm±10 nm in H2O (free dye)

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

fluorescence

-

fluorescence

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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