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64010

Sigma-Aldrich

Metanil Yellow

for microscopy (Hist.), indicator (pH 1.2-2.3)

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Synonym(s):
3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36
Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein:
3831568
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Hist.)
indicator (pH 1.2-2.3)

form

powder

solubility

methanol: 0.01 g/10 mL, orange to very deep orange

εmax

≥500 at 409-419  nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Photocatalytic degradation of azo dye Metanil Yellow: optimization and kinetic modeling using a chemometric approach.
Applied Catalysis. B, Environmental, 77, 1-11 (2007)
An improved stain for connective tissue cells in plastic-embedded sections.
Laboratory Medicine, 10, 783-785 (1979)
Cellular localization of latent murine cytomegalovirus.
Koffron AJ, et al.
Journal of Virology, 72, 95-95 (1998)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et al.
Biotechnic & Histochemistry, 66, 169-169 (1991)
Rais Ahmad et al.
Bioresource technology, 101(10), 3787-3790 (2010-01-26)
In this study, a combined anaerobic-aerobic treatment process based on mixed culture of bacteria was used to degrade azo dyes (AY-36). The experiment was integrated by exposing anaerobic granular sludge and aerobic aromatic amine degrading bacterial enrichment cultures. Under anaerobic

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