Phalloidin–Atto 647N

Name: Phalloidin–Atto 647N
Brand: SIGMA
Price: 549 USD

BioReagent, suitable for fluorescence, ≥80% (HPLC)

Synonym(s):

Atto 647N, Atto 647N-Phalloidin

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10 NMOL

$549.00

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About This Item

NACRES:

NA.32

UNSPSC Code:

12171501

Manufacturer/tradename:

ATTO-TEC GmbH

Key Documents

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Quality Level

100

product line

BioReagent

assay

≥80% (HPLC)

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol

UV absorption

λ: 640-646 nm A_max

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

Related Categories

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

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This Item	07373	05152	49409
Sigma-Aldrich 65906 Phalloidin–Atto 647N	Sigma-Aldrich 07373 Phalloidin–Atto 740	Sigma-Aldrich 05152 Atto 647N DPPE	Sigma-Aldrich 49409 Phalloidin-Atto 488
manufacturer/tradename ATTO-TEC GmbH	manufacturer/tradename ATTO-TEC GmbH	manufacturer/tradename ATTO-TEC GmbH	manufacturer/tradename ATTO-TEC GmbH
product line BioReagent	product line BioReagent	product line -	product line BioReagent
assay ≥80% (HPLC)	assay ≥90.0% (HPLC)	assay ≥90.0% (HPLC)	assay ≥90% (HPLC)
λ in methanol	λ -	λ in chloroform/methanol (8:2)	λ in water
UV absorption λ: 640-646 nm A_max	UV absorption λ: 739 -745 nm A_max	UV absorption λ: 643-649 nm A_max	UV absorption λ: 499-505 nm A_max
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence

General description

Phalloidin–Atto 647N is a new fluorescentlabel targeting the red spectral region. Atto 647N is a cationic dye, and postcoupling, it carries a net electrical charge of +1.Like most Atto labels, the absorption andfluorescence of Atto 647N are independent of pH between 2-11. Atto 647N issupplied in the form of a mixture containing two isomers having identicalfluorescence and absorption properties. Atto labels have rigid structures thatdo not show any cis-trans-isomerization.

Application

Phalloidin–Atto 647Nis designed to be used for labelling DNA, RNA, or proteins. Fluorescentconjugates of phalloidin are used to label actin filaments for histologicalapplications. Some structural features of phalloidin are required for thebinding to actin.

Features and Benefits

Characteristic features of the Phalloidin Atto488 are:

StrongAbsorption.
HighFluorescence quantum yield.
HighPhotostability.
MinimalTriplet formation.
GoodSolubility.
Excellent Ozone Resistance.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

pictograms

GHS06

signalword

Danger

hcodes

H300 + H310 + H330

pcodes

P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Use of the viral 2A peptide for bicistronic expression in transgenic mice.

Georgios Trichas et al.

BMC biology, 6, 40-40 (2008-09-17)

Transgenic animals are widely used in biomedical research and biotechnology. Multicistronic constructs, in which several proteins are encoded by a single messenger RNA, are commonly used in genetically engineered animals. This is currently done by using an internal ribosomal entry

The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin.

Catherine Pfefferli et al.

Nature communications, 8, 15151-15151 (2017-05-04)

The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which

Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.

Benedetta Artegiani et al.

Nature cell biology, 22(3), 321-331 (2020-03-04)

CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. Here, we describe CRISPR-Cas9-mediated homology-independent organoid transgenesis (CRISPR-HOT), which enables efficient generation

An apical MRCK-driven morphogenetic pathway controls epithelial polarity.

Ceniz Zihni et al.

Nature cell biology, 19(9), 1049-1060 (2017-08-22)

Polarized epithelia develop distinct cell surface domains, with the apical membrane acquiring characteristic morphological features such as microvilli. Cell polarization is driven by polarity determinants including the evolutionarily conserved partitioning-defective (PAR) proteins that are separated into distinct cortical domains. PAR

Control of tissue morphogenesis by the HOX gene Ultrabithorax.

Maria-Del-Carmen Diaz-de-la-Loza et al.

Development (Cambridge, England), 147(5) (2020-03-04)

Mutations in the Ultrabithorax (Ubx) gene cause homeotic transformation of the normally two-winged Drosophila into a four-winged mutant fly. Ubx encodes a HOX family transcription factor that specifies segment identity, including transformation of the second set of wings into rudimentary

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