MilliporeSigma
All Photos(1)

66540322

Sigma-Aldrich

PFIZi013-A

Human iPS Cell Line

Synonym(s):
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC

biological source

human dermis (fibroblast)

Quality Level

reprogramming method

Sendai virus

description

age (N/A)

manufacturer/tradename

EBiSC

gender

female

growth mode

adherent (pluripotent)

technique(s)

cell culture | stem cell: suitable

relevant disease(s)

amyotrophic lateral sclerosis (ALS)

shipped in

dry ice

storage temp.

−196°C

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Pfizer Limited - Pfizer

Cell Line Description

Derivation
Primary cell type: Fibroblast
Passage number reprogrammed: 4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown

Characterization
Marker expression: POU5F1 (OCT-4)(+)SSEA-1(-)SSEA-4 (+)TRA 1-60(+)
Differentiation potency:Ectoderm: HES5(+)NeuroD1(+)PAX6(+),EndodermGATA6 (+)SOX17 (+),Mesoderm: DCN(+)MIXL1(+)VIM (+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: No autosomal or sex chromosome aneuplodies were detected
Karyotyping method: BoBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Linkage

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C

Legal Information

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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