MilliporeSigma
All Photos(1)

66540327

Sigma-Aldrich

SIGi001-A-9

Human iPS Cell Line

Synonym(s):
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC

biological source

human epithelium

Quality Level

reprogramming method

retrovirus

description

age (20-24)

manufacturer/tradename

EBiSC

gender

female

growth mode

adherent (pluripotent)

technique(s)

cell culture | stem cell: suitable

relevant disease(s)

corticobasal degeneration

shipped in

dry ice

storage temp.

−196°C

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Sigma-Aldrich

Cell Line Description

Derivation
Primary cell type: Epithelium
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Unknown

Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4(+)TRA 1-60(+)SSEA-1(-)
Differentiation potency:Ectoderm: PAX6 (+),Endoderm: GATA6 (+),Mesoderm: AFP(+)

Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Genetic Modification
Disease/phenotype related modifications
Disease: Corticobasal degeneration
Type of modification: Isogenic
Gene: MAPT
Chromosome location: 17q21.31
Target locus modification: Mutated
Description: P301S+IVS10+16 C>T; Regarding homozygosity/heterozygosity-
Disease: Progressive supranuclear palsy
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms

Linkage

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C

Legal Information

EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service