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MilliporeSigma

68221

Abberior® STAR 440SXP, NHS ester

for long Stokes STED and 2-color STED application

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352108

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Product Name

Abberior® STAR 440SXP, NHS ester, for long Stokes STED and 2-color STED application

assay

≥80.0% (degree of coupling)

form

solid

mol wt

Mw 597.5 g/mol

solubility

DMF: 1 mg/mL, clear

fluorescence

λex 440 nm; λem 511 nm±10 nm in PBS, pH 7.4

storage temp.

−20°C

Quality Level

100

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This Item
383610300430558
Sigma-Aldrich

Sigma-Aldrich

68221

Abberior® STAR 440SXP, NHS ester

Sigma-Aldrich

Sigma-Aldrich

38361

Abberior® STAR 440SXP, maleimide

Sigma-Aldrich

Sigma-Aldrich

03004

Abberior® STAR 512, maleimide

Sigma-Aldrich

Sigma-Aldrich

30558

Abberior® STAR 635, NHS ester

fluorescence

λex 440 nm; λem 511 nm±10 nm in PBS, pH 7.4

fluorescence

λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4

fluorescence

λex 512 nm; λem 530 nm±5 nm in PBS, pH 7.4

fluorescence

λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4

solubility

DMF: 1 mg/mL, clear

solubility

DMF: 1 mg/mL, clear

solubility

DMF: 0.25 mg/mL, clear

solubility

DMF: 0.25 mg/mL, clear

form

solid

form

-

form

-

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

≥80.0% (degree of coupling)

assay

≥80.0% (degree of coupling)

assay

-

assay

-

Application

  • Abberior® STAR 440SXP goat anti-mouse antibody has been used for STED (stimulated emission depletion) microscopy imaging of primary hippocampal cells.[1]
  • Secondary anti-mouse antibody conjugated with Abberior® STAR 440SXP has been used for gated STED microscopy imaging of viral capsid.[2]
  • Abberior® STAR 440SXP goat anti-rabbit antibody has been used for two-color STED microscopy imaging of olfactory epithelium.[3]
Designed and tested for fluorescent super-resolution microscopy

General description

Absorption Maximum, λmax: 433 nm (MeOH)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)

Other Notes

Find more infomation here Abberior®

Legal Information

abberior is a registered trademark of Abberior GmbH

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4474
BCCH0298
BCCD2974
BCBX4190
BCBM9490V

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Linda Westin et al.
BMC neuroscience, 15, 45-45 (2014-03-29)
Norbin is a neuron-specific, cytosolic protein that interacts with the metabotropic glutamate receptor 5 (mGluR5) and has a profound impact on mGluR5 signaling. Yet, little is known about its synaptic distribution. Here we have analyzed the spatial relationship between Norbin
I-Hsuan Wang et al.
Cell host & microbe, 14(4), 468-480 (2013-10-22)
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome
Bastian Henkel et al.
Chemical senses, 40(2), 73-87 (2014-12-17)
Vertebrates can sense and identify a vast array of chemical cues. The molecular machinery involved in chemodetection and transduction is expressed within the cilia of olfactory sensory neurons. Currently, there is only limited information available on the distribution and density
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
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