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68268

Sigma-Aldrich

Imidazole buffer Solution

BioUltra, 1 M in H2O

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Synonym(s):
Imidazole solution, Glyoxaline solution
Empirical Formula (Hill Notation):
C3H4N2
CAS Number:
Molecular Weight:
68.08
Beilstein:
103853
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

form

liquid

concentration

1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

9.5±0.5

density

1.01 g/mL at 20 °C

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

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This Item
1.370988.142234015
Imidazole buffer Solution BioUltra, 1 M in H2O

Sigma-Aldrich

68268

Imidazole buffer Solution

Premium Grade
Imidazole EMPROVE® EXPERT ACS

SAFC

1.37098

Imidazole EMPROVE® EXPERT ACS

-
Imidazole for synthesis

Sigma-Aldrich

8.14223

Imidazole

-
Imidazole ULTROL® Grade, ≥99% (1H-NMR), useful in pH range 6.2-7.8

Millipore

4015

Imidazole

-
form

liquid

form

solid

form

solid

form

solid

concentration

1 M in H2O

concentration

-

concentration

-

concentration

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

-

impurities

-

impurities

-

pH

9.5±0.5

pH

10.5 (20 °C, 67 g/L in H2O)

pH

10.5 (20 °C, 67 g/L in H2O)

pH

-

density

1.01 g/mL at 20 °C

density

-

density

1.233 g/cm3 at 20 °C

density

-

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Application

Imidazole buffer solution has been used as a component:
  • of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
  • of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
  • of imidazole wash and elution buffer for protein purification

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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