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68360

Sigma-Aldrich

Fluorescent Red Mega 485 NHS-ester

BioReagent, suitable for fluorescence, ≥90% (coupling to amines)

Empirical Formula (Hill Notation):
C29H33N3O9S
CAS Number:
Molecular Weight:
599.65
MDL number:
PubChem Substance ID:

product line

BioReagent

Quality Level

Assay

≥90% (coupling to amines)

form

powder

fluorescence

λex 482 nm; λem 559 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CCCCCC(=O)ON1C(=O)CCC1=O)c2ccc3C=C(C(=O)Oc3c2)c4cc[n+](CCCS([O-])(=O)=O)cc4

InChI

1S/C29H33N3O9S/c1-2-31(15-5-3-4-7-28(35)41-32-26(33)10-11-27(32)34)23-9-8-22-19-24(29(36)40-25(22)20-23)21-12-16-30(17-13-21)14-6-18-42(37,38)39/h8-9,12-13,16-17,19-20H,2-7,10-11,14-15,18H2,1H3

InChI key

VKRRBXLGPVGMGA-UHFFFAOYSA-N

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889977506853048
product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

form

powder

form

powder

form

powder

form

-

fluorescence

λex 482 nm; λem 559 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 380 nm; λem 407 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Application

Fluorescent Red Mega 485 NHS-ester is an amine conjugating fluorescent biolabel that can be used for covalent coupling to proteins and other biomolecules containing primary amino groups.Fluorescent red Mega 485 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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