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68652

Sigma-Aldrich

Atto 633 iodoacetamide

suitable for fluorescence

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NACRES:
NA.32

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 629 nm; λem 655 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for coupling to thiols
suitable for fluorescence

storage temp.

−20°C

General description

Atto 633 belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labelling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, good water solubility, and very little triplet formation. Atto 633 is a cationic dye. After coupling to a substrate the dye carries a net
electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications.
The iodoacetamide derivative reacts, like the maleimide, with a sulfhydryl group forming a thioether bond. It is predominantly used for tagging cystein residues of proteins.

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Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Maria Strianese et al.
Protein and peptide letters, 18(3), 282-286 (2010-09-23)
A new, fast, simple and cost-effective sensing device for monitoring H(2)S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H(2)S detection are
Ronny Schmidt et al.
Journal of proteome research, 10(3), 1316-1322 (2011-01-21)
Based on a single-molecule sensitive fluorescence-linked immunosorbent assay, an analytical platform for the detection of lipoarabinomannan (LAM), a lipopolysaccharide marker of tuberculosis, was established that is about 3 orders of magnitude more sensitive than comparable current ELISA assays. No amplification
Thomas D Lazzara et al.
Journal of colloid and interface science, 366(1), 57-63 (2011-10-29)
Anodic aluminum oxide (AAO) substrates with aligned, cylindrical, non-intersecting pores with diameters of 75 nm and depths of 3.5 or 10 μm were functionalized with lipid monolayers harboring different receptor lipids. AAO was first functionalized with dodecyl-trichlorosilane, followed by fusion
Waldemar Waldeck et al.
International journal of medical sciences, 8(2), 97-105 (2011-02-01)
Fluorescent proteins (FPs) are established tools for new applications, not-restricted to the cell biological research. They could also be ideal in surgery enhancing the precision to differentiate between the target tissue and the surrounding healthy tissue. FPs like the KillerRed
Benjamin Strauss et al.
Nucleic acids research, 40(2), 861-870 (2011-09-16)
Chemical probing is a common method for the structural characterization of RNA. Typically, RNA is radioactively end-labelled, subjected to probing conditions, and the cleavage fragment pattern is analysed by gel electrophoresis. In recent years, many chemical modifications, like fluorophores, were

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