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MilliporeSigma

70540

Naphthol Yellow S

suitable for microscopy

Synonym(s):

2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt

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About This Item

Empirical Formula (Hill Notation):
C10H4N2Na2O8S
CAS Number:
846-70-8
Molecular Weight:
358.19
EC Number:
212-690-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329762555
MDL number:
MFCD00003958
Colour Index Number:
10316
Beilstein/REAXYS Number:
3839220

Key Documents

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form

powder

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water, ≥≥ 250 at 387-397 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Show Differences

1 of 4

This Item
49547A553322680
Naphthol Yellow S suitable for microscopy

Sigma-Aldrich

70540

Naphthol Yellow S

Naphthol Yellow S analytical standard

Supelco

49547

Naphthol Yellow S

Alizarin Red S certified by the BSC

Sigma-Aldrich

A5533

Alizarin Red S

Quinoline Yellow suitable for microscopy, mixture of mono- and disulfonic acid sodium salt

Sigma-Aldrich

22680

Quinoline Yellow

grade

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

grade

analytical standard

grade

certified by the Biological Stain Commission

grade

for microscopy (Hist.)

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
food and beverages
personal care

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

solubility

-

solubility

H2O: 1 mg/mL

solubility

H2O: 0.1 g/10 mL

form

powder

form

-

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.[1]

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.[2][3]

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H317,H373

Hazard Classifications

Skin Sens. 1 - STOT RE 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
WE276745/1
276745/1
0000026057
0000027025

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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
Cellular dehydrogenase assay with a tridensity-automicrophotometer.
M Yamada et al.
Cellular and molecular biology, 29(3), 261-266 (1983-01-01)
J James et al.
Cell and tissue research, 243(1), 165-169 (1986-01-01)
With regard to the protein content, as analysed cytophotometrically, of hepatocytes from rats kept under a 12L 12D photoperiod (photophase 7:00-19:00), the following facts have been established: 1) Hepatocytes of different classes of ploidy all demonstrate, more or less equally
N W Schipper et al.
Laboratory investigation; a journal of technical methods and pathology, 61(2), 228-234 (1989-08-01)
The paper describes an improved segmentation method to measure the percentages of epithelium and stroma in ovarian (tumor) tissue with automated image analysis and evaluates its prognostic value. In the image processing method, a blue-yellow image pair is recorded from
Brodsky VYa et al.
European journal of histochemistry : EJH, 37(3), 199-206 (1993-01-01)
Two-wavelength scanning cytophotometry was performed for the measurement of DNA (Feulgen reaction) and total protein content (naphthol yellow S staining) in the same cardiac myocyte. A lack of proportionality between the DNA content (i.e, cell ploidy) and protein content has
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