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70540

Sigma-Aldrich

Naphthol Yellow S

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

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Synonym(s):
2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt
Empirical Formula (Hill Notation):
C10H4N2Na2O8S
CAS Number:
Molecular Weight:
358.19
Colour Index Number:
10316
Beilstein:
3839220
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Hist.)
for the precipitation (of amino acids and peptides)

form

powder

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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This Item
A553322680861286
Naphthol Yellow S for microscopy (Hist.), for the precipitation (of amino acids and peptides)

Sigma-Aldrich

70540

Naphthol Yellow S

Alizarin Red S certified by the Biological Stain Commission

Sigma-Aldrich

A5533

Alizarin Red S

Quinoline Yellow for microscopy (Hist.), mixture of mono- and disulfonic acid sodium salt

Sigma-Aldrich

22680

Quinoline Yellow

Orange G certified by the Biological Stain Commission, Dye content 80 %

Sigma-Aldrich

861286

Orange G

form

powder

form

powder

form

powder

form

powder

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

solubility

H2O: 1 mg/mL

solubility

H2O: 0.1 g/10 mL

solubility

H2O: 1 mg/mL

εmax

≥275 at 423-433 nm in water, ≥≥ 250 at 387-397 nm in water

εmax

-

εmax

≥350 at 283-293 nm in water, ≥450 at 407-417 nm in water

εmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1 - STOT RE 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
W M Frederiks et al.
Histochemistry, 68(1), 49-53 (1980-01-01)
The purpose of this study is to compare the protein content of parenchymal and non-parenchymal nuclei, as isolated from rat liver. The nuclei have been separated by means of a 1 g-sedimentation technique. The protein content of the separated nuclei
J Tas et al.
Acta histochemica. Supplementband, 20, 69-73 (1979-01-01)
After staining with Naphthol Yellow S (NYS) at optimal conditions of pH (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye
Brodsky VYa et al.
European journal of histochemistry : EJH, 37(3), 199-206 (1993-01-01)
Two-wavelength scanning cytophotometry was performed for the measurement of DNA (Feulgen reaction) and total protein content (naphthol yellow S staining) in the same cardiac myocyte. A lack of proportionality between the DNA content (i.e, cell ploidy) and protein content has
J Gaub
The Histochemical journal, 13(5), 717-722 (1981-09-01)
Owing to the accumulation of nuclear non-histone protein (NHP) (a) in cells entering the cell cycle from the quiescent state and (b) in continuously cycling cells during G1 phase, a simultaneous determination of DNA and nuclear NHP is of high

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