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71183

Sigma-Aldrich

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

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Synonym(s):
Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt
Linear Formula:
CH3COONa
CAS Number:
Molecular Weight:
82.03
Beilstein/REAXYS Number:
3595639
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
39021906
PubChem Substance ID:
NACRES:
NA.25

grade

for luminescence
for molecular biology

Quality Level

product line

BioUltra

assay

≥99.0% (NT)

form

solid

autoignition temp.

1112 °F

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

loss

≤0.5% loss on drying, 150 °C

pH

8.5-10.0 (25 °C, 3 M in H2O)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤10 mg/kg
Zn: ≤5 mg/kg

SMILES string

[Na+].CC([O-])=O

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

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Application

Sodium acetate is suitable for use as a buffer in:
  • protein crystallization
  • staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
  • the preparation of standard (acetylcholine chloride) for hestrin assay
  • substrate screening characterization for microfluidic microbial fuel cell array

Biochem/physiol Actions

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Other Notes

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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O-acetylation of typhoid capsular polysaccharide confers polysaccharide rigidity and immunodominance by masking additional epitopes
Hitri K, et al.
Vaccine (2019)
Microfluidic microbial fuel cell array for multiplexed long-term parallel analysis of microbial activities
Hou H, et al.
15th international conference on miniaturized systems for chemistry and life sciences, Seattle, Washington (2011)
Determination of the G+ C content of prokaryotes
Mesbah NM, et al.
Methods in Microbiology (2011)
Enzyme Crystals and Hydrogel Composite Membranes as New Active Food Packaging Material
Mirabelli V, et al.
Global challenges (Hoboken, NJ) (2018)
Large- and small-scale phenol extractions.
D M Wallace
Methods in enzymology, 152, 33-41 (1987-01-01)

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