71376

Sodium chloride

BioUltra, Molecular Biology, ≥99.5% (AT)

• Synonym(s): Salt, Halite, NaCl
• Linear Formula: NaCl
• CAS Number: 7647-14-5
• Molecular Weight: 58.44
• Beilstein/REAXYS Number: 3534976
• EC Number: 231-598-3
• MDL number: MFCD00003477
• UNSPSC Code: 12352302
• PubChem Substance ID: 57652294
• NACRES: NA.25
• assay: ≥99.5% (AT)
• grade: Molecular Biology
• form: solid
• solubility: H₂O: 1 M at 20 °C, clear, colorless

Properties

• grade: Molecular Biology
• Quality Level: 200
• vapor pressure: 1.33 (1.00 mmHg) at 865 °C hPa
• product line: BioUltra
• assay: ≥99.5% (AT)
• form: solid
• availability: not available in (Sales restrictions may apply)
• technique(s): mass spectrometry (MS): suitable; western blot: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.001% total nitrogen (N)
• pH: 5.0-8.0 (25 °C, 1 M in H₂O)
• mp: 801 °C (lit.)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• density: 2.16 g/cm³ at 25 °C (77 °F)
• anion traces: bromide (Br^-): ≤50 mg/kg; hexacyanoferrate(II) ([Fe(CN)₆]^4-): ≤1 mg/kg; iodide (I^-): ≤10 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤200 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: [Na+].[Cl-]
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.01; λ: 280 nm A_max: ≤0.01
• suitability: suitable for component for culture media; suitable for molecular biology
• application(s): genomic analysis
• InChI: 1S/ClH.Na/h1H;/q;+1/p-1
• InChI key: FAPWRFPIFSIZLT-UHFFFAOYSA-M

Description

General description

Sodium chloride, an essential nutrient, plays a critical role in biochemistry and molecular biology, making significant contributions to various biochemical applications. Within biological systems, it serves as a fundamental electrolyte, regulating osmotic pressure, pH balance, and electrical conductivity, all of which are essential for proper cellular function and vital physiological processes. Furthermore, sodium chloride plays a pivotal part in maintaining protein structure and promoting enzymatic activity, essential for stabilizing protein conformations and catalyzing biochemical reactions. Its role extends to the regulation of cellular homeostasis and the facilitation of ion and nutrient transport across cell membranes.It is an integral component of buffers like PBS and SSC, where it aids in various biochemical procedures, including the creation of density gradients and the fine-tuning of biological functions. Notably, sodium chloride is used to increase the ionic strength in buffers and culture media, impacting the ionic environment crucial for specific experiments and cell culture. Moreover, it is applied in DNA precipitation from samples containing SDS and the removal of small nucleic acid fragments during plasmid DNA preparations. Its utility extends to cell culture applications, where it plays a role in bacterial, yeast, and cell culture growth media.In molecular biology and cell culture, sodium chloride is an indispensable salt that finds its place in various aspects of research. It is a common constituent in cell culture media, lysis buffers, and protein storage solutions, contributing to the maintenance of optimal conditions for cell growth and experimentation. The sodium chloride used in these applications is of high purity, rigorously tested for impurities such as DNases, RNases, phosphatases, proteases, and trace metals, making it the ideal choice for molecular biology and biochemical research

Application

Sodium chloride has been used:

• as a component of buffer solution to prepare the pH solutions for open-circuit potential and cyclic voltammetry[1]
• in the preparation of Bold’s basal medium (BBM)[2]
• as a component of transcription and annealing buffer[3]
• to maintain osmotic balance and ensure the purity and stability of the isolated extracellular vesicles (EVs)[4]
• as a buffer for the lamination of lateral flow strips in the fabrication of lateral flow devices[5]

Biochem/physiol Actions

Sodium chloride helps to stimulate the stable induction of T-helper cell 17 (TH17) cells.[6]

Features and Benefits

• Suitable for molecular biology and biochecmial research
• Free from DNase, RNase, NICKase and protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
• Tested for the presence of Anionic and Cationic traces

Other Notes

For additional information on our range of Biochemicals, please complete this form.

This product is intended for research purposes only, and it is not meant for human consumption.

Component of lysis buffer and extraction buffer in the large-scale phenol extraction of RNA[7]; Simple salting out procedure for extracting DNA from human nucleated cells[8]

Sales restrictions may apply

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)